Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Circulation Journal》 2004-06
Add to Favorite Get Latest Update

Cardiac Biological Autonomic Pacemaker Restored by Autologous Transplantation and Committed Differentiation of Bone Marrow Mesenchymal Stem Cells

Ren Xiaoqing, Pu Jielin, Zhang Shu, et al. Cardiac Arrhythmia Center, Cardiovascular Institute and Fu Wai Hospital, CAMS and PUMC, Beijing (100037)  
Objective: To restore cardiac autonomic pace function by autologous transplantation and committed differentiation of bone marrow mesenchymal stem cells, and explore the technique for the treatment of sick sinus syndrome. Methods: Mesenchymal stem cells isolated from canine bone marrow were culture expanded and differentiated in vitro by 5 azacytidine. The models of sick sinus syndrome in canines were established by ablating sinus node with radio frequency technique. Differentiated mesenchymal stem cells labeled by BrdU were autologous transplantated into sinus node area through direct injection. The effects of autologous transplantation of mesenchymal stem cells on cardiac autonomic pace function in sick sinus syndrome models were evaluated by electrocardiography, pathologic and immunohistochemical staining technique. Results: There was distinct improvement on pace function of sick sinus syndrome animal models while differentiated mesenchymal stem cells were auto transplanted into sinus node area. Mesenchymal stem cells transplanted in sinus node area were differentiated into similar sinus node cells and endothelial cells in vivo, and established gap junction with native cardiomyocytes. Conclusion: The committed induced mesenchymal stem cells transplanted into sinus node area can be differentiated into analogous sinus node cells and improve pace function in canine sick sinus syndrome models.
【Fund】: 国家自然科学基金 (30 370 567);; 中国博士后科学基金(2 0 0 2 0 32 1 2 8);; 高校博士点基金资助课题
【CateGory Index】: R541.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved