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Isolation and Identification of Porcine Reproductory and Respiratory Syndrome(PRRS) Virus from aborted fetuses suspected of PRRS

Guo Baoqing(Harbin Veterinary Research Institute,the Chinese Academy of Agricultural Sciences)  
Porcine alveolar macrophages and Marc-145 cells were used to isolate-the porcine repro-ductory and respiratory syndrome(PRRS)virus in pig herds with PRRS outbreaks. Four cy-topathogenic agents were isolated from 4 aborted fetuses, and were identified with PRRS monoclonal antibody SDOW-17 indirect fluorescent antibody(IFA) staining as PRRSV. The 4 isolates ,designated as CH-la , CH-lb,CH-lc , CH-ld .could replicate in both procine alveolar macrophages and Marc-145 cells,and cause cytopathognic effects(CPE)on the infected cells, Preliminary results of IFA staining with antisera to American strains of VR-2332 and NVSL indicated that the 4 isolates were antigenically similar to the American type of PRRS viruses. The 4 isolates did not react with HCV,PrV,PPV,TGEV,PEDV nd HEV antisera in the IFA tests. This is the first report on the isolation of PRRSV in China.
【CateGory Index】: S852.4
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