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《Chinese Journal of Preventive Veterinary Medicine》 2003-04
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Isolation of Mycoplasma galliseticum and detection of the minimum inhibitory concentration for some antibiotics in vitro

WU Qing_min 1,YANG Xiu_yu 2,SHEN Zhi_qiang 3,ZHANG Zhong_zhi 1 (1.Preventive Veterinary laboratory of China Agricultural University,Beijing 100094,China; 2.National Control Insitute of Veterinary Bioproducts and Pharmaceuticals,Beijing 100081,China; 3.Institute of Animal Husbandry and Veterinary Medicine,Binzhou County,Shangdong 251900,China)  
strains of Mycoplasma galliseticum are isolated and identified in chickens from different areas.By assaying for the minimum inhibitory concentration(MIC) of antibiotic in vitro,we find that the mean value of MIC of tiamulin,tylosin,kitasamycin,roxithromycin,lincomycin/spectinomycin,ofloxacin,erythromycin lactate and lincomycin hydrochloride for the 4 strains are 0.062 μg/ml、 0.0171 μg/ml、0.0682 μg/ml、8.5248 μg/ml、8.5248 μg/ml、0.1024 μg/ml、0.4550 μg/ml and 15.4944 μg/ml,respectively.Meanwhile the MICs of different strain of Mycoplasma galliseticum are different to be found.This study shows that Mycoplasma galliseticum easily produce drug_resistant strain and tiamulin,tylosin,kitasamycin and ofloxacin can be used in the recent veterinary clinicd therapy and prevention,while MICs of erythromycin and roxithromycin used routinely for Mycoplasma infection in clinic are found to be high.
【Key Words】: mycoplasma galliseticum isolation and identification the minimum inhibitory concentration
【CateGory Index】: S858.31
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