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《Chinese Journal of Preventive Veterinary Medicine》 2004-01
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Isolation and characterization of porcine rotavirus

CHEN Shu-hong , WANG Xin-sheng , SHI Dong-fang , LI Yi-jing (College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China)  
Porcine rotavirus was successfully isolated from diarrheal piglet fecal samples from Jilin Province. MA104 cells were used for the replication and isolation of the virus. After four generations , the strain could produce obvious cytopathic effects (CPE). Typical rotavirus particles were seen with direct electron microscope. Its RNA electropherotype was 4:2:3:2 which accords with the electropherotype of group A rotavirus. Its characteristics of hemagglutination and plague were studied tentatively. The strain of rotavirus was designated JL94.
【Fund】: 黑龙江省十五攻关课题(项目编号:GC01B510).
【CateGory Index】: S852.65
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