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《Chinese Journal of Preventive Veterinary Medicine》 2004-01
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The research of gold-immunochromatography for CPV diagnostic

WANG Zhong-li~(1), SONG Xiao-hui~(2), CHEN Xi-zhao~(3), CHEN Guo-sheng~(1) 1. National Animal Husbandry & Veterinary Service,Beijing 100026,China; 2.China Agricultural University College of Veterinary Medicine, Beijing 100094 China; 3.Minisry of Agricultural National Veterinary Diagnostic Center ,Beijing 100094 China)  
To develop a rapid, simple and self-performing gold immunochromatography assay (GICA) for the diagnostic of CPV. Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol. Colloidal golds were coupled with canine parvovirus monoclonal antibody, and then canine parvovirus multiclonal antibody was solidified to nitrocellose membrane to prepare a kit. 120 sample of dejection were detect for CPVAg by GICA and HA. The HA titer was above 1:40, the GICA result is positive. The repetition was 100% after the kit was stored six months later. It was confirmed that CPV's GICA was a sensitive, specific , rapid method to detect CPVAg and could be very useful in surveillance of CPV.
【CateGory Index】: S854.4
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