Isolation and identification of a field strain of Marek's disease virus
ZHANG Yan-ping, LIU Chang-jun, SHI Wei-song, QIN Yun-an, ZHANG Xiao-wei, LI Jing-mei (Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China)
A Marek's disease (MD) virus (MDV) strain was isolated in Jilin province from diseased chickens that had been vaccinated by turkey herpesvirus. The isolate was adapted to grow in chick embryo fibroblasts (CEF). Artificial infection of SPF chickens with the virus produced typical clinical symptoms of MD. The isolate was highly pathogenic and caused 76 % and 72 % morbidity in unvaccinated and HVT-vaccinated chickens, respectively. Analysis of 132 bp repeat sequence and meq gene sequences of the viral genome showed that the virus isolate belongs to the highly virulent MDV strains.
【CateGory Index】： S858.31