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《Chinese Journal of Preventive Veterinary Medicine》 2015-02
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Prokaryotic expression of interferon-bata1 of sika deer and the activity identification

SU Feng-yan;LI Zhe;LIU Cun-fa;ZONG Ying;ZENG Fan-li;WANG Quan-kai;College of Chinese Medicinal Materials, Jilin Agricultural University;Wild Animal Rescue and Breed Center of Jilin Province;Jinlin Sino-ROK Academy of Animal Sciences;  
To express the sika deer interferon bata1(IFN-β1), the IFN-β1 gene was amplified by PCR from genome DNA extracted from liver of sika deer, cloned and sequenced. The results showed the sequence of sika deer IFN-β1 gene was 498 bp,which encodes 165 amino acid residues. Then, the IFN-β1 gene was inserted into p ET-28 a vector to construct recombinant plasmid of p ET-IFNβ1. p ET-IFNβ1 was transformed into E.coli Rosseta(DE3). The recombinant IFN-β1(r IFN-β1) was expressed by IPTG inducing. The expressed r IFN-β1 was about 24 ku and mainly in the form of inclusion body which was account for 59.02% of the total bacteria protein. The r IFN-β1 was further purified by the Ni column and renatured. Then, cytopathic inhibition method detected that r IFN-β1 possess antiviral activity which was about 10 ×53.81U/0.1 m L. This study provided a basis for further study and application of sika deer IFN-β1.
【Fund】: 吉林省自然科学基金(201115194);; 国家国际科技合作专项(2011DFA32900)
【CateGory Index】: S825
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