Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Preventive Veterinary Medicine》 2015-02
Add to Favorite Get Latest Update

Prokaryotic expression of interferon-bata1 of sika deer and the activity identification

SU Feng-yan;LI Zhe;LIU Cun-fa;ZONG Ying;ZENG Fan-li;WANG Quan-kai;College of Chinese Medicinal Materials, Jilin Agricultural University;Wild Animal Rescue and Breed Center of Jilin Province;Jinlin Sino-ROK Academy of Animal Sciences;  
To express the sika deer interferon bata1(IFN-β1), the IFN-β1 gene was amplified by PCR from genome DNA extracted from liver of sika deer, cloned and sequenced. The results showed the sequence of sika deer IFN-β1 gene was 498 bp,which encodes 165 amino acid residues. Then, the IFN-β1 gene was inserted into p ET-28 a vector to construct recombinant plasmid of p ET-IFNβ1. p ET-IFNβ1 was transformed into E.coli Rosseta(DE3). The recombinant IFN-β1(r IFN-β1) was expressed by IPTG inducing. The expressed r IFN-β1 was about 24 ku and mainly in the form of inclusion body which was account for 59.02% of the total bacteria protein. The r IFN-β1 was further purified by the Ni column and renatured. Then, cytopathic inhibition method detected that r IFN-β1 possess antiviral activity which was about 10 ×53.81U/0.1 m L. This study provided a basis for further study and application of sika deer IFN-β1.
【Fund】: 吉林省自然科学基金(201115194);; 国家国际科技合作专项(2011DFA32900)
【CateGory Index】: S825
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 QU Jia-qi et al(School of Food and Bio-engineering,Jiangsu University,Zhenjiang,Jiangsu 212013);Research Progress of Interferon Beta[J];Journal of Anhui Agricultural Sciences;2013-09
2 DU Rui1, YIN Mo-lin1, SHI Kun1, ZHENG Xin2, LAN Hai-nan2 (1. College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China; 2. College of Animal Science, Jilin Agricultural University, Changchun 130118, China);Preparation and identification of specific monoclonal antibody against Sika bovine viral diarrhea virus[J];Chinese Journal of Preventive Veterinary Medicine;2010-08
3 ZHANG Yi-yuan,ZHANG Ting-hong,CHANG Wei-shan(Preventive Veterinary Medicine,College of Animal Science and Technology,Shandong Agriculture University,Tai'an,Shandong 271000,China);Cloning,Sequence and Genetic Evolution Analysis of Mink β-interferon Gene[J];Chinese Journal of Veterinary Drug;2012-01
Chinese Journal Full-text Database 9 Hits
1 LIU Yun-long,CHENG Yuan-guo,LIU Xue-long(Agricultural College of Yanbian University,Longjing,Jilin,133400,China);Progress on Interferon[J];Progress in Veterinary Medicine;2008-02
2 LIU Ying 1,2,LIU Dong-guang1,2,LIAO Juan-hong3,YU Qing-long 1,2,XIONG Jia-jun 3,YANG Li-guo3,CHEN Huan-chun 1,2,GUO Ai-zhen 1,2(1.The State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan,Hubei,430070,China;2.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,Hubei,430070,China;3.College of Animal Science and Technologe,Huazhong Agricultural University,Wuhan,Hubei,430070,China);Cloning and Expression of IFN-γ from Cervus nippon and Its Antiviral Activity Analysis[J];Progress in Veterinary Medicine;2009-11
3 XU Hui,LIU De-hui,GUO Xiao-feng(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China);Research Progress of Pig Interferon-beta[J];China Animal Husbandry & Veterinary Medicine;2009-12
4 LI Yijing,WU Yu,TANG Lijie,GE Junwei,QIAO Xinyuan,CUI Wen,JIANG Yanping(School of Veterinary Medicines,Northeast Agricultural University,Harbin 150030,China);Preparation of rabbit antisera against CaIFN-γ and evaluation of CaIFN-γ bioactivity[J];Journal of Northeast Agricultural University;2012-12
5 SU Feng-yan,ZONG Ying,WEI Ji-xiang,ZENG Fan-li,WANG Quan-kai(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun,Jilin,130118,China);Cloning and Phylogenetic Analysis of Interferon-α Gene of Sika Deer[J];Progress in Veterinary Medicine;2013-02
6 LI Yi;WANG Chun-mei;WANG Han-min;DING Qian-nan;DONG Ling;DAI Jun-dong;Beijing University of Chinese Medicine;;Protective effect of 3 different protease inhibitors on the proliferation of velvet antler[J];China Journal of Traditional Chinese Medicine and Pharmacy;2014-10
7 SU Fengyan;LI Zhe;LIU Chunfa;ZONG Ying;ZENG Fanli;WANG Quankai;College of Chinese Medicinal Materials,Jilin Agricultural University;Wild Animal Rescue and Breed Center of Jilin Province;Jinlin Sino-ROK Academy of Animal Sciences;;Cloning and phylogenetic analysis of interferon-β1 gene in sika deer[J];Animal Husbandry & Veterinary Medicine;2014-01
8 LUO Liyong;Tong Qiao hospital of Zhongkai high and new technology Industrial Development Zone of Huizhou City;;Clinical observation of topical interferon combined with microwave in the treatment of 68 patients with condyloma acuminatum[J];China Health Industry;2014-03
9 SU Feng-yan;LI Zhe;LIU Cun-fa;ZENG Fan-li;ZONG Ying;WANG Quan-kai;College of Chinese Medicinal Materials,Ji Lin Agricultural University;Wild Animal rescue and Breed center of Jilin Province;Jinlin Sino-ROK Academy of Animal Sciences;Jiaohe forestry experimental area of administration in Jilin province;;Study on Cloning and Prokaryotic Expression of Interferon-α Gene of Sika Deer[J];Chinese Journal of Veterinary Drug;2015-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XIA Lun-bin1,2,WANG Xin-hua2,LIAN Hong-jun2,BO Xin-wen2,ZHONG Fa-gang2 (1.College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang,832000,China;2.Key Laboratory of Sheep Breeding Biotechnology,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi,Xingjiang,832000,China);Progress on IFN-γ and Its Application in the Prevention and Control of Animal Diseases[J];Progress in Veterinary Medicine;2007-05
2 SONG Xiao-hong1,TANG Jian-hua1,2,WU Jun-wei1,2(1.Chongqing Fangtong Animal Pharmaceutical Co.,LTD,Chongqing Rongchang 402460,China;2.Department of Veterinary Medicine,Southwest University,Chongqing Rongchang 402460,China);Application of interferon in the veterinary clinic[J];Sichuan Animal & Veterinary Sciences;2010-07
3 YANG Qi XIA Chun * ZHAO De-Ming WANG Ming (College of Animal Medicine, China Agricultural University, Beijing 100094, China);Cloning and Expression of Canine Interferon-γ Gene in Mouse SP2/0 Cell Line[J];Chinese Journal of Biotechnology;2002-03
4 XU Hong Fa 1) , LU Hou Ji 1) , SHENG He Lin 1) , GU Chang Ming 2);Status and current distribution of South China Sika Deer[J];CHINESE BIODIVERSITY;1998-02
5 ZOU Yong, SONG Jiping et al( Lanzhou Institute of Biological Products, Lanzhou 730046);Immunogenicity and Curative Effect of Rabies Vaccine-Interferon Combined Preparation[J];Chinese Journal of Biologicals;2002-05
6 Wang Zhonghai (Shenyang Drugs Fast Examination Center,Shenyang,Liaoning,China 110013);Adjuvant Effect Research of Interferon in Rabies Vaccine[J];China Pharmaceuticals;2009-24
7 WANG Yan, WANG Hai-zhen, CAO Rui-bing, CHEN Pu-yan ~(**) (Key laboratory of Animal Diseases Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China );High Level Expression and Activity Assay of Canine Interferon a Gene[J];Virologica Sinica;2005-02
8 HUANG Dao-chao1,2, ZHANG Zhi-he1, YANG Guang-you1,2, ZHANG An-ju1, GUO Wan-zhu2, WANG Cheng-dong1, HOU Rong1, SHEN Fu-jun1, YUAN Xin2 (1. Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China; 2. Key Laboratory for Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya'an 625014, China);Cloning and sequence analysis of IFN-γ gene in Ailuropoda melanoleuca[J];Chinese Journal of Preventive Veterinary Medicine;2008-01
9 WEN Yu-mei Department of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China;Basic and applied studies on the vaccines against hepatitis B virus.[J];Chinese Journal of Hepatology;2003-09
10 WU Yan-hua1,2,WANG Ming1,WU Zhi-guang1(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;2.Section of Laboratory Animals,Capital University of Medicine,Beijing 100069,China);Molecular cloning,expression and antiviral activity analysis of CaIFN-γ gene of German shepherd dog[J];Chinese Journal of Veterinary Medicine;2008-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved