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《Chinese Journal of Preventive Veterinary Medicine》 2016-11
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Expression in MDBK cell and delection of the anti-bovine viral diarrhea virus activity of Sika deer interferon beta1

LIU Qian-hui;LI Zhe;SU Feng-yan;ZENG Fan-li;WANG Quan-kai;Chinese Medicinal Materials College, Jilin Agricultural University;Sika Deer production and application laboratory, Jilin Agricultural University;  
In order to express Sika deer interferon beta1(IFN-β1) and detect the antioxidant activity of bovine viral diarrhea virus(BVDV), the IFN-β1 gene was amplified by PCR from genome DNA extracted from liver of sika deer liver. Then, the IFN-β1 gene was cloned into pVAX1 vector to construct recombinant plasmid of pVAX1-IFNβ1. The pVAX1-IFNβ1 was transfected to Madin-Darby bovine kidney(MDBK) cells. The results show protein was expressed correctly in MDBK cells transfected with pVAX1-IFNβ1 by western blot and indirect immunofluorescence assay. The recombinant proteins was about 25 ku. MDBK cells transfected with pVAX1-IFNβ1 had anti-BVDV activity by cytopathic inhibition method. The study provided a basis for antiviral mechanism and antiviral agent development.
【Fund】: 吉林自然科学基金项目(201115194);; 国家国际科技合作专项(2011DFA32900)
【CateGory Index】: S852.65
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