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《China Pharmacy》 2007-20
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Improvement of HPLC-Fluorometric Method in the Determination of Telmisartan in Human Plasma

WANG Mingxia,HOU Juan,WU Junsheng,ZHANG Junzhen,DU Wenli(The Forth Hospital,Hebei Medical University,Shijiazhuang 050011,China)WANG Min,LI Weiwei(Pharmaceutical College,Hebei Medical University,Shijiazhuang 050017,China)  
OBJECTIVE:To improve the HPLC-fluorometric method in the determination of telmisartan in human plasma.METHODS:Using naproxen as internal standard,the plasma was extracted with ethyl acetate.The separation was performed on Symmetry C8 with mobile phase consisted of acetonitrile -0.01mol·L-1 potassium dihydrogen phosphate buffer solution(47∶53) at a flow rate of 1.0ml·min-1.The λEx was set at 305nm and the λEm was set at 365nm,and the sample size was 20μL.RESULTS:The linear range of telmisartan was 2.5~200ng·mL-1(r=0.999 8),with the lowest detectable limit at 1ng·mL-1.The average recovery of extraction was (89.91±10.07)%.Both the intra-day RSD and inter-day RSD were less than 10%.The sample showed a good stability within 24 hours after three freeze-thaw cycles and extractions.CONCLUSI-ON:The method is simple,sensitive,accurate and reproducible,and suitable for the determination and pharmacokinetic study of telmisartan in human plasma.
【CateGory Index】: R96
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