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《China Pharmacy》 2010-11
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Content Determination of Triterpenoid in the Roots of Acitinidia deliciosa from Guangxi by HPLC

LIANG Jie,ZHEN Han-shen,LI Sheng-mao,LI Wu-lai,ZHANG Wei-wei(School of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning 530001,China)  
OBJECTIVE:To establish an HPLC method for the determination of triterpenoid in the roots of Actinidia deliciosa from Guangxi. METHODS: With the content of 2α,3α,24-trihydroxyursa-12-en-28-oic acid used as index. The separation was performed on Thermo Hypersil BDS C18 column (250 mm×4.6 mm,5 ?m) with 2α,3α,24-trihydroxyursa-12-en-28-oic acid as standard substance. The mobile phase consisted of methanol-0.2% phosphoric acid (73 ∶ 27) with column temperature set at 20 ℃. The flow rate was set at 1.0 mL·min-1 and detection wavelength was 210 nm. RESULTS: The linear range of 2α,3α,24-trihydroxyursa-12-en-28-oic acid were 0.025~0.200 mg·mL-1(r=0.999 8). The average recovery was 99.79%(RSD=1.54%,n=6). CONCLUSION: The method is accurate, reliable and reproducible for the quality control of the roots of A. deliciosa from Guangxi.
【Fund】: 国家自然科学基金资助项目(30160095);; 广西教育厅科研项目(200810LX107);; 广西高校人才小高地建设创新团队资助计划;; 广西中医学院高层次、研究生导师科研项目立项课题(G2006047)
【CateGory Index】: R284.1
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