Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Isolation and Identification of Salvianolic Acid D and Simultaneous Determination of 4 Salvianolic Acids in Danshen Infusion

DING Ning,ZHENG Yun-feng,CHENG Jian-ming,LI He-min,PENG Guo-ping(Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China)  
OBJECTIVE:To establish the method for the isolation and identification of salvianolic acid D and simultaneous determination of tanshinol,protocatechuic aldehyde,salvianolic acid D and B in Danshen infusion.METHODS:RP-HPLC method was adopted for isolation and content determination.The determination was performed on Kromasil C18 column with mobile phase consisted of acetonitrile-1% glacial acetic acid at the flow rate of 1.0 mL·min-1.The injection volume was 10 μL and column temperature was 30 ℃.The detection wavelength was set at 281 nm.RESULTS:Salvianolic acid D had been succeddfully isolated and indentified.The linearities of tanshinol,protocatechuic aldehyde,salvianolic acid D,salvianolic acid B were 60.9~975.0,8.7~139.0,11.0~175.8,2.7~43.2 μg·mL-1.The average recoveries rate were 99.1%,100.2%,98.3%,100.4%,102.5%,101.1%,101.4%,97.0%,102.1%,100.6%,99.2%,101.3%,and the RSDs were 0.94%,0.97%,2.78%,1.07%.CONCLUSION:The method is simple,rapid and specific.It may be suitable for the quality control of Danshen infusion.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved