Toxic Effects of Cadmium on Estrogen Receptor and HSP90 α of Ovary and Uterus Expression in Female Rats
HUANG Ya-qing~,ZHANG Wen-chang,WANG Jia-li,et al.~Center for Disease Prevention and Control of Dongguan City,Dongguan,Guangdong 523016,China
Objective This work was designed to analyze the cadmium effects on expression of estrogen receptor and HSP90 α in both the ovary and the uterus in rats,and to determined mechanisms of sex hormone-disruption of cadmium. Methods 40 female rats were weighed,weight-ranked,and assigned randomly to four groups and were dosed subcutaneously with CdCl_2 at doses of 0,1.25,2.5,and 5.0 mg Cd~(2+)/(kg·d),5 days/wk,for 4 weeks.The rats were killed on the day of the diestrus stage during the 4 day period after administration.Expression of ER and HSP90 α in both ovary and uterus were observed.Results Intensity of ER immunostaining in stroma of ovaries of the 5.0 mg Cd~(2+)/kg group were significantly decreased compared with other groups((P0.05).)Intensity of ER immunostaining in endometria of the CdCl_2 administration groups were not significantly different from the control group(P0.05).Intensity of ER immunostaining in endometria of the 5.0 mg Cd~(2+)/kg group and the 2.5 mg Cd~(2+)/kg group were significantly different from the 1.25 mg Cd~(2+)/kg group(P0.05).Intensity of HSP90 α immunostaining in endometria of the CdCl_2 administration groups were not significantly different from the control group(P0.05).Conclusion The role of ER in the mechanism of endocrine disruption of cadmium might be worth of furter in study in the future.Cadmium can not increase the intensity of HSP90 α expression in endometria,so increasing the intensity of HSP90 α expression in endometria is not one of the mechanisms of endocrine disruption of cadmium in rats.
【CateGory Index】： R114