Biochemical Studies of Chinese Rhubarb ⅩⅩⅩⅡ Organism-Protecting Actions of Rheum Hotaoense Polysaccharide
Yao Wenbing, Chen Qionghua Division of Biochemistry
Rheum hotaoense polysaccharide (RHP) markedly inhibited the growth of tumor S180, with the doses of ig 150 and 300 mg/kg, and the percentages of inhibition were 40% and 48% respectively. RHP showed a remarkable antifree-radical effect on ~(60)Co radiation in mice, with the doses of ig 100 and 200 mg/kg, the mortalities were reduced by 30% and 35% and the average survival times were increased by 26% and 39% respectively as compared with control group. RHP showed a remarkable antagonistic action to leukopenia and the increase of frequency of micronuclei caused by cyclophophamide, with the doses of ig 100 and 200 mg/kg; The antagonistic rates were 33% and 51%, 16% and 32% respectively. RHP reduced the blood glucose level of normal mice and alloxan-induced diabetic mice. With the doses of ig and ip 100 and 200 mg/kg, the blood glucose level was reduced by 20.6% and 43.8% in normal mice, and 53.2% and 57.9% in alloxan-induced mice respectively. With the doses of ip 100 and 200 mg/kg, RHP had antiinflammatory effect on the hindpad swelling in mice induced by carrageenan, the percentages of inhibition were both 78%. RHP, with the doses of ip 100 and 200 mg/kg, also exhibited a remarkable inhibition of the increased SGPT caused by carbon tetrachloride, and the percentage of inhibition were 30% and 32% respectively. The acute LD_(50) of RHP by ip administration in mice was 885.6±128.1 mg/kg.