ISOLATION CULTURE OF ROTAVIRUS AND COMPARING STUDY OF THE DETECTING METHODS
Zhou Zhengren, et al Dept of Microbiology
Twenty-six strains of HRV have been isolated from the feces of 51children with infantile diarrhia. The positive rate is 50.98%. The detectingmethods used here include reverse indirect hemagglutination, direct electronmicroscope examination and HRV-RNA electrophoretic analysis. The resultsshowed that the direct electron microscope examination took the highest positiverate (77.84%) and could be used clinically. Being simple and quick, thereverse indirect hemagglutination (56.9% ) is of importance in distinguishingfrom bacterial gastroenteritis. The finding of 1 short type and 25 long type ofthese 26 strains in HRV- RNA electrophoresis is probably due to the differenceof the type or the antigenicity of the HRV epidemic in the same area andwithin the same time.