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《Journal of China Medical University》 2005-05
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Polymorphism of human cytomegalovirus UL141 gene in clinical isolates of low passage

QI Ying,RUAN Qiang,MA Yan-ping,HE Rong,JI Yao-hua,SUN Zhengrong,HUANG Yu-jing,LIU Qing,CHEN Shu-rong,WANG Ji-dong (Virus Laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China)  
Objective: To study the polymorphism of human cytomegalovirus(HCMV) UL141 in clinical isolates and find the relationship between the polymorphism and the outcome of congenital HCMV infection.Methods: PCR was performed to amplify the entire UL141 region of clinical isolates,which had been proven HCMV-DNA positive by real time PCR.The PCR products were sequenced and the data were analyzed.Results:There was a nucleotide deletion at the position 227 in clinical isoltes,and 2 new UL141 open reading frames named UL141A and UL141B were found.In clinical isolates the predicted proteins of UL141A were hypervariable post the position 75 of amino acid sequence.The predictied protein of UL141B was highly conserved.Conclusion:There are two UL141 open reading frames in clinical isolates.
【Fund】: 国家自然科学基因资助项目(30170986)
【CateGory Index】: R373;
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