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《Journal of China Medical University》 2006-01
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Polymorphism of human cytomegalovirus UL135 gene in low passage clinical isolates

SUN Zheng-rong,RUAN Qiang,HE Rong,MA Yan-ping,QI Ying,JI Yaohua(Virus Laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China)  
Objective: To investigate the polymorphism of human cytomegalovirus(HCMV) UL135 gene in the low passage clinical isolates and to study the relationship between the polymorphism and diseases caused by congenital HCMV infection.Methods: The entire HCMV UL135 gene regions of 29 clinical isolates in which positive HCMV-DNA was detected by using fluorescence quantitative polymerase chain reaction(FQ-PCR) were amplified by polymerase chain reaction(PCR).Amplified PCR products were sequenced,and the sequence were analyzed.Results: Of 29 isolates,25 were amplified successfully.The length of UL135 open reading frame(ORF) in these 25 clinical isolates was similar to that of Toledo sequence.The nucleotide mutation rate of UL135 protein was 0.1% to 2.6%.Additional or deleted sites of posttranslational modification of UL135 protein were found in all clinical isolates.Conclusion: All DNA and deduced amino acid sequences of UL135 gene shared great similarity among HCMV clinical isolates regardless of their polymorphism.No correlation was found between the polymorphism of UL135 gene and the diseases caused by congenital HCMV infection.
【Fund】: 国家自然科学基金资助项目(30170986)
【CateGory Index】: R373
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