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《Journal of China Medical University》 2007-01
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Expression and significance of GAP-43 gene during differentiation of hippocampus-derived neural stem cells in rats

Lü Gang,WANG Yan-feng,HUANG Tao,GAO Da-xin,SUN Ying-fei (Department of Orthopedics,The First Affiliate Hospital,China Medical University,Shenyang 110001,China)  
Objective:To investigate the expression and significance of GAP-43 gene during the differentiation of hippocampus-derived neural stem cell (NSC) in rats. Methods:NSC was obtained from the hippocampus of newborn rats and identified by nestin immunocytochemistry. The expressions of GAP-43 mRNA and protein were determined by reverse transcriptase polymerase chain reaction and immunohistochemical method at different time points during the differentiation. Results:The expression of GAP-43 mRNA was not detected in the neurosphere,expressed at a low level at the 12th hour of differentiation,reached the peak on day 3,and then decreased on day 5. The expression of GAP-43 protein was located in the cytoplasm of NSC on day 1,reached the peak and was mainly located in the prominence on day 5,and decreased gradually on day 7. Conclusion:GAP-43 plays an important role in the growth of prominence during the early period of differentiation of NSC into mature neurons.
【Fund】: 辽宁省自然科学基金资助项目(20052096)
【CateGory Index】: R329
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1 TANG Guang-xu;Hexi University;Beijing Sports University;;The Influence of Long-Term Participation in Sports on QRS Wave Group' Time Limit of Mankind ECG——taking the Sport Department of He Xi University as a example[J];体育科技;2015-02
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