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《JOURNAL OF CHINA MEDICAL UNIVERSITY》 1998-06
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Detection of HBV DNA in Mononuclear Cells from Peripheral Blood among Patients with Hepatitis B Virus Infection

Li Xinming, Li luping, Liu Jun, et al (Department of Microbiology, School of Basic Medical Sciences, China Medical University, Shenyang, 110001)  
Objective:This paper was to study the presence of HBV DNA in mononuclear cells from peripheral blood (PBMCs) among subjects infected with hepatitis B virus and its relationship with serum HBV DNA. Methods:PBMCs and serum samples were examined by PCR and Dot blot hybridization in 50 patients with HBsAg(+) in serum. Results:By PCR,HBV DNA was detected in PBMCs from 35 of 50 patients(70%). By dot blot hybridization, we found HBV DNA in 32 (64.0%). With respect to different types of clinical Hepatitis B, the positive rate of HBV DNA in PBMCs was 60.0%. The positive rate of HBV DNA in serum was 36.0%. Conclusions:HBV DNA could exist in PBMCs independently. We found no obvious difference in the positive rate of HBV DNA from PBMCs among all kinds of clinical hepatitis B.
【CateGory Index】: R512.620.2
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