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《Chinese Journal of Oil Crop Scieves》 2001-01
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Genetic diversity of Brassica napus L. accessions from China and Czech based on RAPD markers

HU Sheng-wu 1, ZHAO Hui-xian 2, YU Chen-yu 1, J. Ovesna 3, V. Kucera 3, M.Vyvadilova 3, L.Kucera 3 ( 1. College of Agronomy, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China; 2. College of Life Sc  
The genetic diveristy and the relationships among a collection of Brassica napus L. accessions, including 20 Chinese, 25 Czech, 2 Germany, 2 France and 1 England, were evaluated using random amplified polymorphic DNA (RAPD) markers. A total of 79 polymorphic amplified products were obtained from 10 primers. The Hamman metric based distance was chosen for which the best fit between a priori and a posteriori grouping was found using UPGMA. Cluster analysis based on Hamman genetic distance placed accessions in the experiment into three main groups. The first group included 16 rape accessions from China and the second group that from Czech with the exception of "Zhongshuang No.2", a Chinese winter rape. The third group included accessions both from China and Czech. There were genetic variation among experiment accessions, however, the genetic variation between Chinese and Czech accessions was greater than that among Chinese accessions and that among Czech accessions. In view of the obvious genetic differences, the Czech B.napus L. accessions would be an important resources for enriching the genetic background to Chinese rapeseed, and vice versa.
【Fund】: 中捷国际合作项目! (34— 16 ) ;; 中国人事部归国留学人员基金;; 陕西省科委攻关课题! (97K0 1—G2— 0 3)
【CateGory Index】: S565.403.53
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