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《Chinese Journal of Oil Crop Scieves》 2002-02
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Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD

DUAN Yuan-sheng, LIU Ping-wu, YANG Guang-sheng ( National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Research Center of National Rapeseed Improvement, Wuhan 430070, China )  
random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.
【Fund】: 国家 8 63项目 (2 0 0 1AA2 41111)
【CateGory Index】: S565.403
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