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《Chinese Journal of Oil Crop Sciences》 2016-02
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Introduction of glyphosat resistant gene into Ricinus communis L. by Agrobacterium mediated transformation using germinating embryos

YANG Jun-fang;WANG Ya;ZHANG Huan-huan;WANG Yi-xue;HAO Yao-shan;GUO Zhi-qiang;WANG Hong-wei;College of Bio - engineering,Shanxi University;The Industrial Crop Institute of Shanxi Academy of Agricultural Sciences;Research Center of Biotechnology,Shanxi Academy of Agricultural Sciences;  
To improve the castor( Ricinus communis L.) gene transformation,imbibed seeds( germinating embryos) were transformed with herbicide resistant gene CP4- EPSPS by Agrobacterium- mediated transformation without tissue culture. Result showed that pricking 3 stitches at meristematic region( apical point) of germinating seeds followed by ultra- sonication( 800 W),and co- cultured with Agrobacterium for 16 h were the optimum transformation conditions. 5 putative transgenic plants were detected from 800 randomly- selected treated seedlings,with average positive rate of 6. 3 ‰. After glyphosate screening,3 positive T0 seedlings were obtained out of26 survivals by PCR and Southern blot. This method was much simpler and more effective than the procedure of tedious tissue culture.
【Fund】: 公益性行业(农业)专项(201003057);; 山西油料作物种质资源创制与研究利用(2015ZZCX-07);; 转基因生物新品种培育重大专项(2014ZX08003001-002-003)
【CateGory Index】: S565.6
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