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Isozyme analysis of sesame core collection and reserve collection

Zhao Yingzhong Cheng Yong Zhang Xiurong Feng Xiangyun Fu Ming Guo Qingyuan ( Oil Crops Research Institute, CAAS, Wuhan 430062 ) Zhou Mingde( IPGRI East Asia Office, Beijing 100081 ) Toby Hodgkin( IPGRI, Rome, Italy )  
The isozyme technology of polyacrylamide gel vertical slab electrophoresis was used to analyze esterase, peroxidase, malate dehydrogenase, 6—phosphoglucoates dehydrogenase and amylase of 36 landraces selected from seven ecological regions of Chinese sesame germplasm resources. The research indicated that only esterase zymograms presented the diversity differences among accessions and within an accession. Total 1080 individuals from nine landraces in core collection and nine in reserve collection originated from three sesame ecological regions of north, central and south China were tested by esterase. The results showed that eight zymogram types were detected out in mobility (Rf) between 0.46 and 0.67. Throuth the comparison of zymogram type frequency, band distribution, varietal diversity index and cluster analysis, the core collection representated the reserve collection well.
【CateGory Index】: S565.303.2
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