Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Pharmaceutical Journal》 2008-21
Add to Favorite Get Latest Update

Study on Extraction of Total Flavonoids in Anoectochilus roburghii by Orthogonal Design

GUAN Jing,WANG Chun-lan,GUO Shun-xing,XIAO Pei-gen(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China)  
OBJECTIVE To study the extraction technology of total flavonoids for Anoectochilus roburghii.METHODS The extraction condition was optimized with orthogonal design by the detection of UV spectrum.RESULTS Flavonoid extraction was affected by ethanol concentration,extraction frequency,extraction time and solvent quantity.CONCLUSION The optimum extractions are A1B3C3D3.The material was soaked overnight with 12 volumns of 95% EtOH,kept circumfluence for 1.5 h,this operation was repeated for 3 times.Average recovery is 98.51%,and RSD is 2.068%.
【Fund】: 国家自然科学基金资助项目(30270148);; 国家杰出青年科学基金资助项目(30325047)
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 ZHAO Chuan, TANG Sheng-an, DUAN Hong-quan(Tianjin Research Center of Basic Medical Science, School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070,China);Study on Extraction of Anti-diabetes Active Constituents from Potentilla chinesis[J];Journal of Chinese Medicinal Materials;2010-02
2 GU Hui-fen1,ZHUANG Yi-1i1,MEI Qi-chun2(1.Shanghai Institute of Chinese Materia Medica,Shanghai 201401,China;2.School of Life Sciences,Fudan University,Shanghai 200433,China);Comparison of effective components and RAPD between wild and tissue culture seedlings of Anoectochilus roxburghii[J];Chinese Traditional Patent Medicine;2011-08
3 TAN Zhi-jian,LI Fen-fang,LIU Tai-ping(College of Chemistry and Chemical Engineering,Central South University,Hunan Changsha 410083,China);Study on the separation and purification of anthraquinone aglycone by polyamide resin[J];Chinese Journal of Hospital Pharmacy;2011-11
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HU Guo-hai et al (Research and Development Center of Biological Resources,Wenshan College,Wenshan,Yunnan 663000);Content Measurement of Microelements in Artificial Anoectochilus roxburghii in Wenshan of Yunnan Province[J];Journal of Anhui Agricultural Sciences;2010-14
2 GU Hui-fen1,ZHUANG Yi-li1,MEI Qi-chun2(1.Shanghai Institute of Chinese Materia Medica,Shanghai 200127,China; 2.School of Life Sciences,Fudan University,Shanghai 200433,China);RAPD Analysis of Tissue Culture Seedlings of Dendrobium candidum and Design of Specific Primer for Identification[J];Journal of Fudan University(Natural Science);2007-03
3 HUANG Li-ying, LIN Xin-hua, LAI Li-Min, ZHENG Yan-jie, LIU Si-yi College of Pharmacy, Fujian Medical University, Fuzhou 350004, China;Determination of Aloe-Emodin and Aloin without Separation by Dual-Wavelength Spectrophotometry[J];Journal of Fujian Medical University;2005-01
4 LIU Run-dong~1,GUO Wen-jie~2,LIN Zhong-ning~1,LU Xue-hua~(2*)(1.Agricultural Ecological Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;2.Biotechnological Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);Tissue culture and nutrient contents analysis of Anoectochilus roxburghii.[J];Guangxi Agricultural Sciences;2006-05
5 HE Tie-guang~(1,2,3),SU Jiang~(2,3),WAN Can-qin~1,LU Jia-shi~1(1.Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;2.Agricultural College,Guangxi Univ.,Nanning 530005,China;3.Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China);A comparison of polysaccharide and amino acid contents of Dendrobium candidum with different resources[J];Guangxi Agricultural Sciences;2007-01
6 WANG Hang(Department of pharmacy;First offiliated haspital of Fujian medical university Fuzhou,350003,China);Hypoglycemic effects of anoectochili active components and its mechanism[J];Strait Pharmaceutical Journal;2009-12
7 CHEN Wen-wen,HUANG Qin-wan,LAN Yan,WU Chun-jie*(Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China);Content determination of bergenin in ethnodrug Rodgersia sambucifolia Hemsl. by HPLC[J];West China Journal of Pharmaceutical Sciences;2007-02
8 LU Cheng-wei(School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001);Research Progress and Protection Utilization of Anoectochilus[J];;2010-08
9 ZHANG Zhi-xin,ZHANG Tie,ZHAO Bao-fa,HU Guo-hai,ZHANG Shi-xiu(Department of Biochemistry,Wenshan Normal College,Wenshan,Yunnan 663000,China);Determination of Total Flavonoids and Polysaccharide of the Wild Anocetochilus Plants in Wenshan Prefecture[J];Lishizhen Medicine and Materia Medica Research;2009-06
10 Feng Weisheng. Zheng Xiaoke (Heuau College of Traditioal Chinese Medicine,Zhengzhou 150003) Yoshida T. and Okuda T. (Faculty of Pharmaceutical Sciences ,0kayama University, 700 Japan);FIVE HYDROLYZABLE TANNINS FROM POTENTILLA DISCOLOR BUNGE[J];Natural Product Research and Development;1996-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved