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Study on Extraction of Total Flavonoids in Anoectochilus roburghii by Orthogonal Design

GUAN Jing,WANG Chun-lan,GUO Shun-xing,XIAO Pei-gen(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100094,China)  
OBJECTIVE To study the extraction technology of total flavonoids for Anoectochilus roburghii.METHODS The extraction condition was optimized with orthogonal design by the detection of UV spectrum.RESULTS Flavonoid extraction was affected by ethanol concentration,extraction frequency,extraction time and solvent quantity.CONCLUSION The optimum extractions are A1B3C3D3.The material was soaked overnight with 12 volumns of 95% EtOH,kept circumfluence for 1.5 h,this operation was repeated for 3 times.Average recovery is 98.51%,and RSD is 2.068%.
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