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Effect of Polysaccharides of Cistanche deserticola on Immune Cells and Telomerase Activity in Aging Mice

ZHANG Hong-quan,LI Yuan,SONG Yuan-yuan(Medical and Pharmaceutical Institute,Yangzhou University,Yangzhou 225001,China)  
OBJECTIVE To study the effect of polysaccharides of cistanche deserticola(PCD) on tissue malondialdehyde(MDA) content and telomerase activity and immune function of experimentally aging model mice.METHODS Subacute aging model mice were induced by D-galactose.The MDA content and telomerase activity were measured with thiobarbituric acid colorimetric method and PCR-ELISA,respectively.The lymphocyte proliferation was measured by MTT method.Phagocytosis of peritoneal macrophages was tested by neutral red test.The interleukin-2(IL-2) content in peripheral blood was measured by ELISA method.RESULTS In model group,MDA content was significantly increased in heart,liver and brain,telomerase activity,lymphocyte proliferation,phagocytosis of peritoneal macrophages and blood IL-2 content were obviously decreased.After treatment with PCD,MDA content was significantly decreased in heart,liver and brain.The telomerase activity in heart and brain,lymphocyte proliferation,phagocytosis of peritoneal macrophages and peripheral blood IL-2 content were obviously enhanced.CONCLUSION PCD can prevent aging of mice induced by D-galactose,which may be related to antagonize the free radical injury and enhance the immunity of aging mice.
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