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《Chinese Journal of Hospital Pharmacy》 2006-06
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Determination of cinobufagin in rat serum by HPLC

ZHANG Lei~(1),QI Gang~(2),ZHANG Li~(3),ZHANG Lian-chun~3,KE Zheng~3,XIONG Xiao-jun~3 (1.Department of Pharmacy,Affiliated Hospital of Medical College of Chinese People's Armed Police Forces,Tianjin 300162,China;2.Central Laboratory of Medical College of Chinese People's Armed Police Forces,Tianjin 300162,China;3.Department of pharmacy,Medical College of CPAP,Tianjin 300162,China)  
OBJECTIVE To establish an HPLC method for determination of cinobufagin in rat serum and to investigate the pharmacokinetics of cinobufagin.METHODS The separation was carried out by a reversed phase VPODS column(150 mm×(4.6) mm,5 μm)with a mobile phase consisting of methanol-water(70∶30,v/v),detection wavelength was 290 nm.35 Wister rats were included in this study.The pharmacokinetics of cinobufagin had been investigated in rats serum after intravenous administration(1.0) mg·kg~(-1).RESULTS The detection limit recovery was(0.05) mg·L~(-1).InterK-day RSD was less than 4% and intra-day RSD less than 10%.The recovery was(81.3%).The calibration curve was liner over range from(0.25) mg·L~(-1) to(8.0) mg·L~(-1).CONCLUSION This method is sensitive,convenient and accurate,and is suitable for the determination in rat serum and pharmacokinetics studies.
【CateGory Index】: R285
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