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《Chinese Journal of Hospital Pharmacy》 2013-06
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Studies on the chemical constituents for antitussive effect in the active ingredients of Verbena officinalis L.

REN Fei1,DUAN Kun-feng2,FU Ying1,WANG Xin3,REN Xiao-dan4(1.Dept.of Pharmacy,The Second Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China;2.Dept.of Pharmacy,The Third Hospital of Hebei Medical University,Hebei Shijiazhuang 050051,China;3.Dept.of Pharmacy,The Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China;4.Dept.of Paediatrics,Hebei Provincial Hospital of Traditional Chinese Medicine,Hebei Shijiazhuang 050011,China)  
OBJECTIVE To study the chemical constituents for antitussive effect in the active ingredients of Verbena officinalis L..METHODS The chemical constituents from Verbena officinalis L.were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography,preparative high performance liquid chromatography(HPLC).Their structures were identified by physicochemical properties and spectral analysis.RESULTS Twelve compounds were isolated and identified as kaempferol(1),apigenin(2),4'-hydroxywogonin(3),quercitrin(4),luteoiin(5),isorhamnetin(6),verbascoside(7),verbenalin(8),hastatoside(9),aucubin(10),gentiopicroside(11),β-sitosterol(12).CONCLUSION isorhamnetin(6) is obtained from Verbena officinalis L.for the first time.
【Fund】: 河北省卫生厅计划项目(编号:20110093)
【CateGory Index】: R284
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