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《Chinese Acupuncture & Moxibustion》 2011-04
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Effects of reinforcing and reducing methods by twirling and rotating the needle on contents of CGRP and NO in rats with stress-induced hypertension

WANG Li1,JING Ming-xin2,ZHI Jian-mei3,LU Jun3,WANG Chao-yang3,Director:LIU Qing-guo3(1.TCM International Cooperation Center of China,Beijing 100101,China;2.National Research Center for Rehabilitation Technical Aids;3.Beijing University of CM,Beijing 100029)  
Objective To explore the different effects of reinforcing and reducing methods by twirling and rotating the needle on stress-induced hypertension and its mechanism.Methods Sixty male Wistar rats were randomly divided into a nomal group(group A),a model group(group B),an acupuncture control group(group C),an acupuncture with reinforcing method group(group D)and an acupuncture with reducing method group(group E),12 rats in each group.The model of stress-induced hypertension was established by foot-shock and noise stimulation in the other groups except group A.Group C was treated by acupuncture at left "Taichong"(LR 3) with no manipulation,group D with twirling reinforcement method and group E with twirling reducing method at the same acupoint,respectively,and group A and B with no acupuncture treatment.The contents of calcitonin gene-related peptide(CGRP) and nitrogen monoxidum(NO) in blood plasma and hypothalamus after 7 days were observed.Results After acupuncture treatment,the contents of CGRP in blood plasma and hypothalamus were(375.9±36.5) pg/mL and(213.6±50.1) pg/mg in group A,which were higher than(261.0±20.1) pg/mL and(156.0±21.8) pg/mg in group B,and(255.3±33.6) pg/mL and(154.3±47.3) pg/mg in group C(all P0.05),and(465.9±53.2) pg/ mL and(250.74±47.7) pg/ mg in group E higher than those in group C and(283.3±29.8) pg/ mL and(164.6±47.4) pg/ mg in group D(allP0.05),and there was no significant difference among group B,C and D(allP0.05).After acupuncture treat ment,the contents of NOin blood plasma and hypothalamus were(43.7±5.5)μmol/Land(23.3±2.9)μmol/gin group B,(46.5±6.4)μmol/Land(23.1±3.3)μmol/gin group C,(45.8±6.7)μmol/Land(25.0±2.7)μmol/gin group D,which were alllower than(64.5±9.9)μmol/L and(30.7±1.6)μmol/gin group A(allP0.05),(55.9±4.6)μmol/L and(30.4±2.1)μmol/g in group E higher than those in group Cand group D(allP0.05),and there was no significant difference among group B,C and Deither(allP0.05).Conclusion Acupuncture with twirling reduc-ing method canincrease the contents of CGRP and NOin rats with stress-induced hypertension,thereby causinga fall in blood pressure,however,no significant influence withtwirling reinforcing method.Reinforcing or reducing method by twirling and rotating the needle has different efficacy.
【Fund】: 国家自然基金课题资助项目:30772833;; 北京中医药大学校级课题资助项目
【CateGory Index】: R245.3
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