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Study of Inhibitory Effect of Quercetin on Neovascilarization of Experimental Mammary Carcinoma

Kong Lingquan Wu Kainan Lin Hui Department of Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing  
Objective: To investigate the inhibitory effect of flavonoids quercetin on neovascularization of experimental mammary carcinoma. Methods: DMBA-induced animal model of mammary carcinoma was produced in rats. Seventy-nine female Sprague-Dawly rats were randomized into four groups: DMBA, DMBA+TAM, DMBA+quercetin and control. Samples of breast of these four groups were collected 28 weeks later, and were observed under light microscope and microvessel density (MVD) estimation. Their expressions of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and the protein product of H-ras were examined by immunohistochemical staining. Results: (1) Mammary Carcinoma incidence rate and mean mammary tumor diameter of group A (76.2%,2.37cm) were significantly higher than those of group B (40.9%,1.82cm), C (45.5%,1.71cm) and D (0%,0cm) (P<0.05), and there was no significant difference to be found between group B and group C (P>0.05). It indicated that quercetin could inhibit the occurrence and growth of mammary carcinoma; (2) MVD estimation and immunohistochemical staining of VEGF,bFGF and H-ras protein product showed significant difference between group A and group B, group A and group C (P<0.05) with no significant difference between group B and group C (P>0.05). Conclusion: Quercetin can liably reduce the incidence and growth of mammary carcinoma induced by DMBA and the following mechanisms may be responsible for this observation: (1) To inhibit the synthesis of H-ras protein so as to inhibit the proliferation of tumor cells and tumor angiogenesis; (2) To inhibit the activities of angiogenesis-related growth factors such as VEGF and bFGF so that the angiogenesis in mammary carcinoma is inhibited with decreased MVD in mammary carcinoma of rats received quercetin treatment.
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