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《Chinese Journal of Osteoporosis》 2013-05
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Effect of aspirin on rat osteoclast differentiation and bone resorption activity

ZHANG Yi1,NIU Erlong1,LI Yang1,LIANG Yuan2,QIN Junhui2, YI Hui3,WANG Ruian2,SANG Hongxun1(Department of Orthopedics,Xijing Hospital;2.Department of Pathology and Pathophysiology; 3.Department of Traditional Chinese Medicine,the Forth Military Medical University,Xi'an,710032,China)  
Objective To investigate the effect of different doses of aspirin on rat osteoclast(OC) differentiation and bone resorption activity in vitro.Methods Rat myelomonocytes were cultured with soluble receptor activator of NF-κB ligand(sRANKL) and macrophage colony stimulating factor(M-CSF).Estrogen(10-6 mmol/L) and aspirin(0.25 mmol/L,0.5 mmol/L,1.0 mmol/L,and 1.5mmol/L) were additioned to the medium.The tartrate-resistant acid phosphatase(TRAP) staining was performed to observe cell morphology.The number of osteoclast-like cells was counted.Osteoclasts were inoculated on bone slices in order to build osteoclast-bone slice analytic model.The bone slices were observed using light microscope and scanning electron microscope at different time.The area of resorption pits was analyzed.Results The number of osteoclasts and pit areas in estrogen group were lower than those in normal control group,and the difference was statistically significant(P0.05).With the increase of aspirin concentrations,the number of TRAP-positive multinucleated osteoclasts and the area of resorption pits gradually decreased to none,and the difference was statistically significant(P0.05).Compared to those in estrogen group,the results in low aspirin concentration group(0.25 mmol/L) had no significant difference.While the number of TRAP-positive multinucleated osteoclasts and the areas of resorption pits gradually decreased in the higher aspirin concentration groups(0.5 mmol/L,1 mmol/L,1.5 mmol/L),and the difference was statistically significant(P0.05).Conclusion Aspirin can inhibit osteoclast differentiation and bone resorption activity in a dose-dependent manner,thus exerting its anti-osteoporosis effect.
【Key Words】: Aspirin Osteoclast Bone resorption Osteoporosis
【Fund】: 国家自然科学基金面上项目(81270959)
【CateGory Index】: R580
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