Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Osteoporosis》 2019-08
Add to Favorite Get Latest Update

The mechanism of Jiangu granule in promoting osteoblast proliferation based on Cyclin D1 knock-down

YANG Juan;JIA Xiaokang;ZHANG Chutian;HUANG Yunmei;HUANG Meiya;ZHANG Zhiheng;SUN Pan;WU Yinsheng;LIN Yanping;Fujian University of Traditional Chinese Medicine;  
Objective To verify the regulation of Cyclin D1 and downstream signaling proteins on osteoblast proliferation cycle and explore the mechanism of Jiangu granule in promoting the proliferation of osteoblasts. Methods The osteoblast-like cell lines UMR-106 was knock-down of the Cyclin D1 gene by CRISPR/Cas9 gene editing and divided into 2 groups: gene knock-down +saline group( group 1) and gene knock-down + Jiangu granule group( group 2). The virus empty vector cell( group 3) was used as negative control. Cells without gene knock-down were divided into normal cells + saline group( group 4) and normal cells + Jiangu granule group( group 5). The cells were respectively treated by saline or Jiangu granule drug-containing serum for 24,48 and 72 hours,and CCK8 method was used to detect cell proliferation. Gene expression of Cyclin D1,Cyclin E,CDK 2,CDK 4,Rb and E2 F-1 were detected by Real time PCR. Results Western-bolt detection showed that the expression of Cyclin D1 protein decreased significantly in osteoblasts with gene knock-down. The result of CCK8 test shows that compared with normal cells + saline group,normal cells + Jiangu granule group's multiplication rate was obviously increased( P 0. 05),negative control group had no significant difference( P 0. 05),while gene knock-down + saline group and gene knock-down + Jiangu granule group had muchslower rate( P 0. 05). Compared with normal cells + Jiangu granule group,there was a significant decrease in gene knock-down +Jiangu granule group( P 0. 01),and there was no significant difference between gene knock-down + saline group and gene knockdown+ Jiangu group( P 0. 05). Real time PCR demonstrated that compared with normal cells + saline group's mRNA expression of Cyclin D1,CDK2,CDK4,Cyclin E,Rb and E2 F-1,the expression in normal cells + Jiangu granule groupwas distinctly increased( P0. 05 or P0. 01),while negative control group had no significant difference( P 0. 05),and the expression in gene knock-down + saline group and gene knock-down + Jiangu granule groups were significantly reduced( P 0. 05 or P 0. 01).Compared with normal cells + Jiangu granule group's,the gene knock-down + Jiangu granule group had decreased rate( P 0. 01),while there was no significant difference between gene knock-down + saline group and gene knock-down + Jiangu group( P 0. 05). Conclusion Cyclin D1 and its downstream signaling proteins are the key factors to regulate the proliferation of osteoblasts. The function of Jiangu granule in promoting osteoblast proliferation may be achieved by regulating Cyclin D1 and its downstream signaling proteins.
【Fund】: 国家自然科学基金(81473706 81574003)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 LI Chong;LV Weihua;WANG Tingting;XING Yurong;ZHANG Lixia;LIU Xiaogan;WANG Zhifang;ZHENG Lili;Department of Endocrinology ,The First Affiliated Hospital of Zhengzhou University;Henan Medical Association;Department of Medical Center,The First Affiliated Hospital of Zhengzhou University;;The study of prevalence of osteoporosis in 1088 medical workers[J];中国骨质疏松杂志;2015-10
2 Jia Ying-min;Li Rui-yu;Wu Mi-shan;Huo Rui-lou;Li Bin;Department of Nephrology,Beijing Military General Hospital;Department of Internal Medicine,Integrated Traditional and Western Medicine Hospital of Pingxiang;Institute for Integrated Traditional and Western Medicine,the Second Affiliated Hospital of Xingtai Medical College;Prescription Staff Room,Hebei College of Traditional Chinese Medicine;Department of Biochemistry,Hebei Medical University;;Effects of different compatibility proportion of active ingredients of Kidney Recipe on osteoblast proliferation and differentiation[J];中国组织工程研究;2015-24
3 Yan Jun-li 1 , Li Meng 2 , Li Rui-yu 3 , Wu Li-ping 4 , Sun Yan-fu 3 , Li Hui-long 3 ( 1 Ren County Hospital, Xingtai 055150, Hebei Province, China; 2 Health Team, Hotan Detachment of the Xinjiang Armed Police Corps, Hotan 848011, Xinjiang Uygur Autonomous Region, China; 3 Institute for Integrated Traditional and Western Medicine, the Second Affiliated Hospital of Xingtai Medical College, Xingtai 054000, Hebei Province, China; 4 Department of Laboratory Medicine, Xingtai People’s Hospital, Hebei Medical University, Xingtai 054031, Hebei Province, China);Chinese herbal compound affects osteoblast proliferation and bone mineral density[J];中国组织工程研究;2013-33
4 XU Xiaojuan1 SHEN Lin1 YANG Yanping1 ZHOU Fanhua1 SHUAI Bo1 Li Chenggang1 WU Manxiang1 MA Chen1 1Department of Integrated Traditional Chinese Medicine and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;The Effect of Qing'e Pill on BMD and Bone Turnover Markers in Patients with Postmenopausal Osteoporosis[J];中国中医骨伤科杂志;2013-06
5 GUAN Xiaoyan,ZHOU Weibing,HUANG Juan,WANG Longyun,LIAO Yuping (Department of Oncology,Xiangya Hospital,Central South University,Changsha 410008,China);Effect of CDK2-AP1 gene over-expression on proliferation and cell cycle regulation of breast cancer cell line MCF-7[J];中南大学学报(医学版);2012-10
6 LIN Haiming1,LIN Yanping2,WU Yinsheng2(1.College of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China; 2.Fujian Academy of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China);Effects of Serum Containing Jiangu Granules on Expression of OPG and RANKL mRNA in Osteoblast-like Cells[J];福建中医药大学学报;2012-01
7 WU Yin-Sheng, LIN Yan-ping,LU Tian-Xiang,et al Osteopathy Institute,Fujian Academy of Integrative Medicine,Fuzhou (350108);Effects of Jiangu Granule Containing Serum on the Cyclins in Rat's Osteoblast at G_1 Phase[J];中国中西医结合杂志;2010-09
8 Wu Yin-sheng1,Lin Yan-ping1,Lu Tian-xiang2,Lin Yu1,Huang Yun-mei3,Huang Mei-ya3 1Institute of Bone Disease,Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,Fujian Province,China;2Department of Orthopaedics,Quanzhou First Hospital,Quanzhou 362000,Fujian Province,China;3Key Laboratory of Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,Fujian Province,China;Changes in G_1 phase regulatory protein of osteoblasts in ovariectomized osteoporosis rats[J];中国组织工程研究与临床康复;2010-15
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YU Xianbin;SUN Liaobin;HU Wei;The Second Affiliated Hospital,Wenzhou Medical University;;Effects of Gukang Capsule on Post Menopausal Osteoporosis Patients and Level of Biochemical Markers of Bone Turnover[J];中华中医药学刊;2015-12
2 WENG Ze-bin;YAN Cui-ping;ZHANG Zhi-jie;GAO Qian-qian;ZHAO Gen-hua;CHEN Zhi-peng;CAI Bao-chang;LI Wei-dong;Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; Pharmacy College of Nanjing University of Chinese Medicine;Taizhou Institute for Food and Drug Control;Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences;;Serum Containing Drug of Different Processed Products of Qing'e Pill on Osteoblast Proliferation,Differentiation and Mineralization[J];中国实验方剂学杂志;2015-06
3 LI Ping;SHEN Lin;YANG Yanping;College of Basic Medicine,Hubei University of Traditional Chinese Medicine;Department of Integrated Traditional Chinese and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology;;Clinical Research of Qing'e Pill in Treatment of Postmenopausal Osteoporosis with Kidney Deficiency and Blood Stasis Syndrome[J];中西医结合研究;2014-06
4 YAN Cui-ping;WENG Ze-bin;WU Yu;CHEN Zhi-peng;CAI Bao-chang;LI Wei-dong;Jiangsu Key Laboratory of Chinese Medicine Processing,Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine;Jiangsu Jumpcan Pharmaceutical Group Co.,Ltd;Pharmacy College,Nanjing University of Chinese Medicine;Pharmacy Department of Nantong hospital of traditional Chinese medicine;;Study on the correlation between the salt-roast processing and the anti-osteoporosis efficacy of Qing'e Pill[J];南京中医药大学学报;2014-05
5 GUAN Jun-sheng;GUO Hai-ling;ZHANG Zi-jia;LI Tao;WANG Zheng-tao;Department of Pharmacognosy,China Pharmaceutical University;Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine;Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine; The MOE Key Laboratory for Standardization of Chinese Medicines; Shanghai Key Laboratory for Compound Chinese Medicine;;Effects of Du-Zhong cortex extract on osteoblast cell proliferation,differentiation and Col1αⅠ expression[J];上海中医药杂志;2014-07
6 CHEN Li-ai;Acupuncture and Massage Department,Hengxian Hospital of Chinese Medicine;;Progress Study in Tonifying Kidney Prescription in Treating Osteoporosis[J];按摩与康复医学;2014-05
7 LIU Jinke;SHEN Lin;YANG YanPing;XU XiaoJuan;Jianghan District Evergreen Street Community Health Center in Wuhan;Department of Tradional Chinese Medicine,Union Hospital affiliated to Tongji Medical College,Huazh-ong university ofScience and Technology;;The Effect of Qing'e Pill on The Serum Levels of IGF-1、IL-6 and TNF-αin Patients with Postmenopausal Osteoporosis Accompanied with Pain on Bone[J];中国中医骨伤科杂志;2014-04
8 Wang Danhui;Ben Yue;Han Mei;Jilin Academy of Traditional Chinese Medical Sciences;;Clinical study of Rana temporaria oil in the treatment of postmenopausal osteoporosis[J];中医正骨;2014-01
9 Feng Wen-cheng;Liang Wei-guo;Fourth Affiliated Hospital of Jinan University;;Proteomics and its application in orthopedic diseases[J];中国组织工程研究;2014-02
10 GAO Shuang-quan;GAO Shuang-rong;XIAO Gao-fang;DING Yu;WANG Lin-hui;HUANG Wei;LI Jing;Department of Pathology, Yue Bei People's Hospital;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;;Correlation of CDK2-AP1 gene over-expression with proliferation and cell cycle regulation of breast cancer cell line MCF-7.[J];海南医学;2013-17
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Ye;TONG Jie;ZHOU Yan-jing;XU Xiao-yu;College of Pharmaceutical Sciences,Institute of Chinese Medicine,the Key Subject Constructing Units of Pharmacology of Chinese Materia Medica by the State Administrative Bureau of Traditional Chinese Medicine,Southwest University;Department of Clinical Pharmacy of the First People's Hospital,Yunnan Province;;Research progress on anti-osteoporotic active ingredients and pharmacological action mechanism of traditional Chinese kidney-tonifying and bone-strengthening drugs[J];中国中药杂志;2015-06
2 ZHANG Xiao;ZHANG Guoqing;GU Bolin;JIANG Pengfei;Department of Rehabilitation,The Second Affiliated Hospital of Suzhou University;;Current advance in the effect of salvia miltiorrhiza and its effective components on bone metabolism[J];中国骨质疏松杂志;2015-01
3 YI Dong-sheng;LIN Lin;GAO Feng;Orthopedic Surgery,the Second People's Hospital of Dalian;Dalian Medical University;;Establish of neonatal rat osteoblasts and research of osteoblasts biological activies[J];大连医科大学学报;2014-05
4 LUO Qiaoyan;YANG Maojun;XU Yong;GAO Chenlin;HUANG Wei;CHEN Guo;ZHOU Xueqin;FENG Hong;GOU Fang;LV Shishi;LIU Shuang;CHEN Yan;CHEN Decai;Department of Endocrinology,the Affiliated Hospital of Luzhou Medical College;Department of Endocrinology,West China Hospital of Sichuan University;;The investigation of prevalence and related risk factors of osteoporosis in women in the upper reaches of the Yangtze River[J];中国骨质疏松杂志;2014-07
5 GAO Lu;ZHENG Hong-xin;CHEN Yi-jing;ZONG Zhi-hong;LIN Shu-ru;Liaoning University of Traditional Chinese Medicine;China Medical University;;Impacts on BMSCs Osteoblast Differentiation and Regulation of RUNX2 and OSX in the Rats with Chinese Herbal Medicine for Tonifying Kidney[J];世界中西医结合杂志;2014-04
6 QIAN Zhenfu;GUO Meiling;HE Xuying;YANG Hongbing;Miyun Chinese Medicine Hospital;;Beijing suburb of different occupations in the distal forearm bone mineral density comparative analysis[J];中国骨质疏松杂志;2014-03
7 CHENG Kui;GE Baofeng;CHEN Keming;Zhen Ping;ZHOU Jian;MA Xiaoni;SONG Peng;MA Huiping;Institute of Orthopedics,Lanzhou General Hospital of Lanzhou Military District;Department of Pharmacy,Lanzhou General Hospital of Lanzhou Military District;;Oral medication of icariin enhances peak bone mineral density and bone quality in rats[J];中国骨质疏松杂志;2014-02
8 Han Ya-jun;Tie Xiao-jia;Yilihamu·Tuoheti;Department of Orthopedics,Second Affiliated Hospital of Xinjiang Medical University;;Meta-analysis on the prevalence rate of osteoporosis in the middle-aged and elderly in China[J];中国组织工程研究;2014-07
9 Cheng Hao;Zhang Yan-fang;Xu Wei;Department of Physics,School of Information Engineering,Guangdong Medical College;Guangxi Wuzhou Pharmaceutical (Group) Co.,Ltd.;;Primary culture and identification of neonatal rat osteoblasts[J];中国组织工程研究;2013-41
10 MA Xiao-ni,GE Bao-feng,CHEN Ke-ming,ZHOU Jian,SHI Wen-gui,XIE Yan-fang, GUO Xiao-yu,L Xiang,CHENG Kui,GAO Yu-hai Institute of Orthopaedics,Lanzhou General Hospital,Lanzhou Command of PLA,Lanzhou 730050,China;Mechanisms of Icariin in Regulating Bone Formation of Osteoblasts and Bone Resorption of Osteoclasts[J];中国医学科学院学报;2013-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved