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The establishment of a method of isolated and cultured chicken osteoclasts

Shi Fengqin; Yu Shifeng; Pang Shuzhen.(Department of oral pathology, School of Stomatology,Beijing medical university, Beijing 100081)  
In this study, in order to obtain large numbers of pure and viable osteoclasts, we improved the method of isolated and cultured rabbit osteoclasts. The osteoclastic population 1 were obtained from 28-day old chicken long bones by flushing the marrow cavities repeatedly. The bones were then treated successively with collagenase and trypsin. The osteoclastic population 2 and population 3were obtained. These isolated cells were cultured on glass of bone slices. Under phase-contrast microscope, it has been found that these isolated multinucleated giant cells can migrate and generate resorption lacunae on bone slices. In addtion, these cells stained positively for acid phosphatase which aremarker for osteoclasts. These results suggested that the procedure of isolation of osteoclasts from thelong bones of chicken was successful. The numbers of chicken osteoclasts are larger obtained by this method than rabbit osteoclasts obtained by old method. The vitality of the chicken osteoclasts are stronger. This method lays a foundation for further study of pathogenesis of bone resorption.
【Fund】: 国家自然科学基金
【CateGory Index】: R329-33
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