Studies on immunoregulation of polysaccharides-1a from Radix Cynanchi Bungei
GAO Li-jun~1, WANG Jian-hua , CUI Jian-hua~(3), WANG Han-zhong~2(1. College of Pharmacy, Taishan Medical University, Taian 271000, China; 2. College of Agronomy, Shandong Agricultural University, Taian 271018, China;3. Department of Chemistry and Chemical Engineering, Taishan Medical University, Taian 271000, China)
Objective: To study the effects of PRCB1a(one component of polysaccharides from Radix Cynanchi Bungei)on transformation of T lymphocytes of rabbit in vitro and immune function in mice. Method: Three doses of PRCB1a (2,4,6 g·L~(-1)) were respectively put in bottle with PHA and blood of rabbit. The effect of PRCB1a on immunity in vitro was studied by observing transformation of T lymphocytes; The dosage of PRCB1a (50,100,150 mg·kg~(-1)·d~(-1)) was given orally for seven days. The effects on immune function were investigated in mice. Result: Three doses of PRCB1a could significantly promote (P0.01) the ability of T lymphocytes proliferation; PRCB1a could improve the mouse thymus and spleen index, the celiac macrophage ability of engulfing CRBC, the delayed type hypersensitivity ability and the macrophage engulfing carbon granula ability. Conclusion: The results indicate PRCB1a can enhance nonspectific and specific cellular immune function.