Estrogenicity of adzuki bean Phaseolus angularis wight.and its effect on proesterone receptor level in human breast cancer MCF-7 cells
ZHANG Xing-guo,ZHAO Qing-wei (First Affiliated Hospital,College of Medicine,Zhejiang University, Hangzhou 310003,China)
Objective: To explore the estrogenic activity of ethanol extract from adzuki bean Phaseolus angularis and its effect on progesterone receptor(PR) level of human breast cancer MCF-7 cells. Method: The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of adzuki bean extract.And its effect on PR mRNA and protein were addressed using RT-PCR measurements and western blotting analysis respectively in which pure estrogen receptor antagonist ICI 182,780 was employed as the tool.Result: The estrogenic activity of adzuki bean extract at various concentrations(10-200 μg·mL~(-1)),expressed as proliferative effect(PE) relative to that of solvent control,was examined.The results indicated that the extract of adzuki bean was able to induce MCF-7 cell growth with a maximum at 100 μg·mL~(-1).The results of RT-PCR and western blotting showed that PR mRNA and protein could be significantly induced by adzuki bean extract in MCF-7 cells.Moreover,the specific estrogen receptor antagonist ICI 182,780 could block these reactions. Conclusion: Ethanol extract of adzuki bean has the estrogen-like activities through the estrogen response pathway.
【CateGory Index】： R285.5