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《China Journal of Chinese Materia Medica》 2006-19
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Studies on extraction,isolation and composition of Lycium barbarum polysaccharides

TIAN Meili,WANG Min(School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China)  
Objectove: To study the extraction,isolation and composition of Lycium barbarum polysaccharides(LBP).Method: LBP was extracted from L.barbarum with water,isolationed and purified by DEAE ion-exchange cellulose and gel chromatography,and their structural composition was studied by means of SDS-PAGE gel electrophoresis,GC,amino acid automatic analysis,etc.Result: Pure LBP has four water solubie polysaccharides,M W was 1.524×10~5.LBP was composed of 6 kinds of monosaccharides(Ara,Rha,Xyl,Man,Gal and Glc),galacturonic acid and 18 kinds of amino acids.Conclusion: LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein,and LBP has Glycan-O-Ser glycopeptide structures.
【Fund】: 黑龙江省教育厅科研资助项目(10533008)
【CateGory Index】: R284.1
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