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《China Journal of Chinese Materia Medica》 2007-03
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Determination of matrine,sophoridine and oxymatrine in compound Kushen injection by HPLC

TIAN Juan1,2,WANG Wei-hao1,GAO Hui-min1,WANG Zhi-min1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.College of Life Science,Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China)  
Objective: To establish a high performance liquid chromatography(HPLC) method for determination of matrine,sophoridine and oxymatrine in Compound Kushen Injection.Method: Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol(80∶10∶10) as the mobile phase and the detection wavelength was at 220 nm.Result: The mean recovery of matrine,sophoridine and oxymatrine in Compound Kushen Injection was 99.51%(RSD 1.58%),99.24%(RSD 1.44%) and 100.22%(RSD 1.85%),respectively.Conclusion: The method was simple,rapid,accurate and specific and suitable for quality control of the product.
【CateGory Index】: R286
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