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《China Journal of Chinese Materia Medica》 2009-14
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Identification on Syngnathus and its adulterants with random amplified polymorphic DNA

WU Yan,LIU Jia,WANG Mengyue,JU Peijun,LI Xiaobo(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)  
Objective: To study the identification method of Syngnathus and its adulterants.Method: Random amplified polymorphic DNA(RAPD) was used to construct a dendrogram by UPGMA method based on Nei Li's coefficient and a genetic affinity pattern for Syngnathus acus,Solenognathus hardwickii,Syngnathoides biaculeatus,Trachyrhamphus serratus,Halicampus koilomatodon,Microphis boaja.Result: Four primers,LJ04,LJ09,LJ16 and LJ19,from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification.The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants.Conclusion: RAPD is suitable to identify Syngnathus and its adulterants.
【Fund】: 国家自然科学基金项目(30672620)
【CateGory Index】: R282.5
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Chinese Journal Full-text Database 10 Hits
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