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《China Journal of Chinese Materia Medica》 2009-14
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Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration

SONG Shihua1,3,JIA Xiaobin1,2,CHEN Yan1,2,WANG Lijing3,FENG Liang3(1.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica,Jiangsu Provincial Academy of Chinese Medicine,Nanjing 210028,China;2.Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine,Nanjing 210028,China;3.School of Pharmacy,Jiangsu University,Zhenjiang 212013,China)  
Objective: To study the feasibility of supercritical fluid extraction(SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.Method: Optimum extraction conditions were studied by orthogonal tests,the ergosterol were analyzed by RP-HPLC.Result: The optimal extraction conditions were pressure as 20 MPa,temperature at 45 ℃,using ethanol as modifier carrier at the volume of 1 mL·g-1,and 1.5 hour of extraction time.Conclusion: SFE has the superiority of adjustable polarity,and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.
【Fund】: 江苏省公益项目(BM2007701)
【CateGory Index】: TQ461
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