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《China Journal of Chinese Materia Medica》 2009-24
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Effects of puerarin on expression of apelin and its receptor of 2K1C renal hypertension rats

JIN Ge1,YANG Penglin1*,GONG Yongsheng2,FAN Xiaofang2,TANG Jifei1,LIN Jiafeng1 (1.Department of Cardiovascular Internal Medicine,Second Affiliated Hospital,Wenzhou Medical College,Wenzhou 325027,China;2.Institute of Cor Pulmonale and Teaching Centre of Functional Experiment,Wenzhou Medical College,Wenzhou 325035,China)  
Objective: To examine the change of puerarin on the expression of apelin and its receptor of the two-kidney,one-clip(2K1C) rats.Method: Tirty male Sprague-Dawley rats were randomly divided into normal control group(C),model group(M) and puerarin group(P).The mean of carotid arterial pressure(mCAP),mean of left ventricular end diastolic pressure(LVEDP),and the weight ratio of left ventricular mass(left ventricle plus septum)to bodyweight(LVM/BW) were measured to evaluate the model of 2K1C renal hypertension.The concentrations of apelin in the plasma and left ventricle(LV) were measured with radioimmunoassay.Apelin mRNA and APJ mRNA expressed in the LV were examined by reverse transcription-polymerase chain reaction(RT-PCR).The peptides of apelin and APJ expressed in the LV were detected with immunohistochemistry(IHC).Result: Compared with C group,the mCAP,LVEDP and LVM/BW of M group were higher 36.58%,333.8% and 20.24%,respectively(P0.05,P0.01,P0.01).Compared with M group,LVEDP and LVM/BW of P group were lower 65.24% and 13.12%,respectively(both P0.05).However mCAP was of no significant difference between these two groups.The levels of apelin-36 in the plasma and LV of M group were respectively higher 18.56% and 207.38% than those of C group(both P0.05),while ones of P group were lower 24.21% and 49.40% than those of M group(both P0.05).The expressions of apelin mRNA and APJ mRNA at left ventricle tissues of 2K1C rats were higher 77.66% and 119.00%(both P0.05) than those of C group.The ones of P group were lower 27.40% and 45.66% than those of M group(both P0.01).The IHC results indicate that the expressions of apelin and APJ peptides at left ventricle tissues of 2K1C rats were higher 129.51% and 154.1%(both P0.01) than those of C group,respectively.Whereas the ones of P group were lower 65.36% and 62.87% than those of M group(both P0.01).Conclusion: Through regulating apelin/APJ system puerarin has protective effect on the development of left ventricular hypertrophy by renal hypertension.
【Fund】: 温州市科技局项目(Y2003A104)
【CateGory Index】: R285.5
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