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《China Journal of Chinese Materia Medica》 2012-23
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Pharmacokinetic study on three main ingredients of refined coronary cataplasm

HOU Rui,WANG Lan2,DU Mao-bo2,LIANG Ri-xin2,LIU Shu-zhi2,WANG Yan-li2,ZHANG Jian-yong2(1.Capital Medical University,Beijing 100069,China; 2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)  
Objective: To establish a LC-MS/MS method for determining the concentration of tanshinone ⅡA,salvianolic acid B and paeoniflorin of refined coronary cataplasm in rabbit plasma,in order to determine the concentration of the three main ingredients in blood after transdermal administration and calculate their pharmacokinetic parameters.Method: Rabbits were given refined coronary cataplasm on the basis of 15 g·kg-1 by transdermal administration to detect the plasma concentration of the three main ingredients using LC-MS/MS.Winnonlin software was used to calculate their major pharmacokinetic parameters.Result: Tanshinone ⅡA,salvianolic acid B and paeoniflorin showed good linearity(r0.999) at 1-100,50-1 000,10-1 000 μg·L-1 respectively in plasma,with average recovery rate of 96.57%,91.90%,95.93%,respectively.The RSD within day were less than 15%.After transdermal administration of refined coronary cataplasm in rabbits,the main pharmacokinetic parameters of tanshinoneⅡA,salvianolic acid B or paeoniflorin were as follows: Cmax(20.85±12.68),(636.25±386.91),(787.80±395.64) μg·L-1;Tmax(0.49±0.28),(0.44±0.27),(0.46±0.30) h.Conclusion: The LC-MS/MS method is highly selective and sensitive to determine the concentration of samples in rabbit plasma.The pharmacokinetic characteristics of tanshinone ⅡA,salvianolic acid B and paeoniflorin are suitable to assess the percutaneous absorption of refined coronary cataplasm.
【Fund】: 国家“重大新药创制”科技重大专项(2009ZX09502);; 中国中医科学院基本科研业务费自主选题项目(ZZ2007046)
【CateGory Index】: R285
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