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《China Journal of Chinese Materia Medica》 2015-04
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Physiological and biochemical change of Paris seed in after-ripening during variable temperature stratification

LI Zhao-ling;TONG Kai;YAN Shen;YANG Hua;WANG Qiao;TANG Yong-bin;DENG Meng-sheng;TIAN Meng-liang;College of Agronomy,Sichuan Agricultural University;Yaan Sanjiu Chinese Herbal Medicine Technology Industry Company;  
In order to explore the dormancy physiological and biochemical mechanism of Paris seeds,the seed embryo growth courses,and the dynamic change of 5 enzymes,include SOD,POD,CAT,MDH,G-6-PDH were measured during variable temperature stratification. The results indicated that Paris seeds embryo grew quickly after 40 d in warm-stratification( 18 ± 1) ℃,at the meantime the metabolic activity was significantly strengthened. These facts showed that Paris seeds turned into physiological after-ripening process.After 60-80 d,the morphological embryo after-ripping process basically completed,and the following cold-stratification( 4 ± 1) ℃ furthered Paris seed to finish physiological after-ripening. After 40 d,the activity of MDH decreased while G-6-PDH increased significantly. This showed that the main respiratory pathway of seed changed from TCA to PPP,which benifited breaking seed dormancy. In the whole period of stratification process,the activity variation of SOD and CAT was insignificantly and the activity of POD was enhanced significantly after shifting the seed in cold stratification process. This showed that SOD,CAT had no direct effects on breaking Paris seed dormancy but keeping the seed vigor,while the POD might involve in the process of Paris seed dormancy breaking.
【Fund】: 国家“十二五”科技支撑计划项目(2011BAI13B02-6)
【CateGory Index】: S567.239
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