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《China Journal of Chinese Materia Medica》 2015-19
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ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum

YANG Lu-cun;LIU He-chun;ZHOU Xue-li;XU Wen-hua;ZHOU Guo-ying;Northwest Institute of Plateau Biology,Chinese Academy of Sciences;Key Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences;University of Chinese Academy of Sciences;Tiebujia Grassland Improvement Experiment Station;  
In this study,31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure. The results showed that: the ITS region ranged in size from 634 to 635 bp and base composition was with high G + C content of57. 8%. Thirty-one polymorphic sites were detected from 402 sequences of 31 populations of N. incisum,and the proportion of polymorphic sites was 4. 88%,in which parsimony informative sites were up to 12. And 31 haplotypes were identified based on these polymorphic sites. Molecular variance analysis( AMOVA) indicated that high genetic differentiation( 57%) existed among population,and gene flow was low( Nm= 0. 38) among populations. Phylogenetic relationships of 31 haplotypes were analyzed using NJ method with N. forbesiias an out-group. Phylogenetic analysis showed that 31 haplotypes from different populations mixed together and did not form distinct geographically separated clades.
【Fund】: 青海省青年基金项目(2013-Z-942Q)
【CateGory Index】: S567.239
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