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《CHINESE JOURNAL OF PATHOLOGY》 1995-03
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Isolation and characterization of human lung cancer cell subline with different metastatic petential

Zhu Weiyong;Zheng Jie;Fang Weigang, et al (Department of Pathology, Beijing Medical University,Beijing l00083)  
ingle cell clones were isolated from metastatic human lung giant cell carcinoma(PG)using limited dilution method。After preliminary screening by in vitro invasion assay,tumorigenicity and spontaneous metastasis assay in nude mice,three cloned PG cell sublines(PGBE_1,PGCL_3 and PGLH_7)were selected for further observation on morphology,chromosome number,in vitrO growth and invasion assys,tu-morigenicity and spontaneous metastasis to lung and lymph nodes in nude mice,We found that there was some difference on spontaneous metastasis to lung and lymph nodes in nude mice and in vitro inva-sive abilities between PGBE_1 and PGLH_7.But there were no obvious differences in other biological fea-tures among the three sublines.The results show that the PG cell line consists of different subpopula-tions of cells with differrent metastatic properties. These sublines may be valuable for further study on the molecular mechanisrns of cancer metastasis。
【Key Words】： Lung neoplasms Neoplasm metastasis Dissection
【Fund】： 国家“八．五”攻关课题，国家自然科学基金
【CateGory Index】： R734.2
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