p53 alterations in human lung cancer and their correlation with clinicopathological features and prognosis

Zhang Jun, Zheng Jie, Fang Weigang,et al. Department of Pathology, Beijing Medical University, Beijing 100083  

Objective To investigate p53 protein accumulations and p53 mutations in squamous cell carcinoma and adenocarcinoma of the lung and their correlation with clinicopathological features and prognosis. Methods Immunohistochemistry (LSAB) and nonisotopic PCRSSCP were used to detect p53 abnormalities in lung cancer. Results The general positive rates for immunohistochemistry using antip53 monoclonal antibody Do7 (DAKO) as first antibody was 50.5% in 95 cases of lung cancere, 565% (26/46) in squamous cell carcinoma and 44.9% (22/49) in adenocarcinoma. PCRSSCP results showed that 39 cases (41.1%) showed abnormal bands. The mutation rate in squamous cell carcinoma was 47.8% (22/46) and 34.7% (17/49) in adenocarcinoma. In 15 (38.5%) cases, the mutations were located on exon 5, 3 (7.7%) were on exon 6, 13 (33.3%) on exon 7 and 8 (20.5%) on exon 8, respectively. The concordance rate of immunohistochemistry and PCRSSCP was 77.9% (74/95). The mutation rates of p53 gene in NO, N1, N2 staged lung cancer were 22.6% (7/31), 44.2% (19/43) and 61.9% (13/21), respectively. The difference was statistically significant (P0.025). The 3 year survival rate in patients with p53 mutations was significantly lower than those without p53 mutations (P0.025). Conclusion The location of p53 mutations in squamous cell carcinoma and adenocarcinoma of the lung were more commonly seen on exon 5 and exon 7. p53 mutations were associated with increased lymph nodes metastasis and reduced survival rate in squamous cell carcinoma and adenocarcinoma of the lung.

【Key Words】： Lung neoplasms Protein p53 Genes p53 Mutation Prognosis
【Fund】： 美国中华医学基金
【CateGory Index】： R734.2

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