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《CHINESE JOURNAL OF PATHOLOGY》 1998-05
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Zymographic analysis of MMP s in human carcinoma cell lines with different metastasis potential

Li Hongmei, Fang Weigang, Zheng Jie, et al. Department of Pathology, Beijing Medical University, Beijing 100083  
Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected. Results The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa′s, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP 9, but primary stage cells don′t. There are MMP 9 in the conditioned medium of prostatic carcinoma cell PC 3M, the metastatic clone of PC 3 which don′t express MMP 9. Conclusion The expression of MMPs especially MMP 9 of carcinoma cells is closely correlated to the metastatic and invasive potential.
【Key Words】: Lung neoplasms Neoplasm metastasis Neoplasm invasiveness Metalloproteinases
【Fund】: 国家自然科学基金
【CateGory Index】: R73-37
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