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《CHINESE JOURNAL OF INFECTIOUS DISEASES》 1998-02
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A novel method to study the full length genome of hepatitis B virus

He Jianwen, He Lifang, Yao Xin, et al. Department of Molecular Virology, Shanghai Medical University, Shanghai 200032  
Objective To study the association between pathogenicity and variants of hepatitis B virus(HBV) by full length HBV genome, a novel method of amplification of the whole genome of HBV strains from serum samples of patients by PCR, followed by enzyme digestion and cloning was established. Methods Two sets of primers were designed: one with SpeⅠ restriction site, the other with restriction sites of SalⅠ and SapⅠ. PCR products were amplified separately from the single stranded and the double stranded regions of the genome, and the two PCR products obtained were 1.15 kb and 2.05kb. After cleavage and ligation, the full length HBV genome was used to transfect HepG2 cell line. Results Expression of viral antigens and intracellular replication of HBV were observed with the full length HBV genome by the novel method. Conclusion This novel method could serve to study the structure and function of HBV genome from viral strains of clinical samples.
【Fund】: 国家自然科学基金
【CateGory Index】: R512.62
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