Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinexe Journal of Pediatrics》 2002-03
Add to Favorite Get Latest Update

Multi-system rotavirus infection in 2 infants

LI Ning, YAO Yingmin. Department of Pediatrics,Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China  
Objective Recently, there are increasing reports on rotavirus infection in extraintestinal organs in infants with diarrhea that may cause severe consequences. Here we report 2 cases with rotavirus infection which involved multiple organ systems for clinical reference. Methods The genome of rotavirus was tested in the tissues obtained from 2 infants died of rotavirus infection. The organs and tissues involved included the brain, heart, lung, spleen, pancreas, intestine, appendix, lymph nodes and kidney. The specimens embedded in paraffin were examined with indirect in situ polymerase chain reaction and hybridization technology. The tissue sections were tested with RT-PCR. The sections were then incubated with hybridization solution with the probe at 42℃ for 18 hours and DAB was added and incubated until positive brown-yellow color appeared. Results The genomes of rotavirus were found in intestine, liver, lung and kidney of the 2 cases. The positive staining was seen in intestinal mucosal villi layer and some of intestinal gland cells both on membrane and in cytoplasm; the lymphocytes beside intestinal gland were positive obviously in membrane and weakly in cytoplasm; no positive result was observed in submucosal layer. In the kidney, epithelial cells of proximal convoluted tubule in renal cortex were positive in cytoplasm, while glomerulus, distal convoluted tubule, collecting tubule, stroma and renal medulla were negative. In the lung, the terminal bronchiole and some alveolar epithelial cells were positive; the stroma was negative. In the liver, many hepatocytes were positive both on membrane and in cytoplasm; several Kupffer cells were obviously positive in cytoplasm. Conclusion The presence of rotavirus in the lung, the liver and the kidney indicates that rotavirus can invade such organs/tissues directly. Viremia was probably the route of transmission of the virus to the various organ systems and lymphatc vessels might also contribute to spread of rotavirus. The appearance of rotavirus in Kupffer cells in the liver might be attributed to phagocytosis.
【CateGory Index】: R446.8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 Li Ning Ou Qiaoqun~① Dai Jing~② et al.Nanfang Hospital,Guangzhou 510515,China ①Gerneral Hospital of Guangzhou Military Area Command,Guangzhou 510010,China. ②Changhai Hospital,Second Military Medical University,Shanghai 200433,China;Exploring the distribution of rotavirus receptors in human organs[J];Infectious Disease Information;2008-04
2 CHEN Chun-hua, REN Xiao-mei, WANG Jian-rong(The 5th Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, China);Clinical Analysis of Involved Organs Besides Gastro-intestine after Rotavirus Infection in Infants and Young Children[J];Endemic Diseases Bulletin;2010-02
3 YAO Ying-min1, OU Qiao-qun1, CHEN Yao2 Department of Pediatrics, Nanfang Hospital1, School of Biotechnology2, Southern Medical University, Guangzhou 510515, China;Ultrastructural changes of the extraintestinal organs of newborn mice with human rotavirus infection[J];Journal of Southern Medical University;2006-09
4 YAO Ying-min1, OU Qiao-qun1, ChenYAO2 1Department of Pediatrics, Nanfang Hospital, 2College of Biotechnologies, Southern Medical University, Guangzhou 510515, China;Factors triggering extraintestinal infection of rotavirus[J];Journal of Southern Medical University;2006-10
5 ;Analysis of Cellular Immunity in Children with Intestinal and Extraintestinal Rotavirus Infection[J];China Foreign Medical Treatment;2010-31
6 Liu Lili 1 Huang Yongkun 2 Wei Qunde 2 , et al. (1. Department of Pediatrics, Beijing General Hospital of Ministry of Coal Industry, Beijing 100028; 2. Department of Pediatrics, The First Affiliated Hospital of Kunming Medical College, Kunming 650032);Detection Rotavirus Genome in the Blood of Children with Rotavirus Diarrhea by Different Kinds of PCR Tests[J];Clinical Medical Journal of China;2004-03
7 Shao Chuanda,Li Xiguang.Pediatrics Department,Yanggu County People’s Hospital. Shangdong,252300,China;The clinical significance on the detection of mycardial enzyme in child-patients with rotavirus enteritis[J];Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease;2005-04
8 NIU Zhi-min(the People's Hospital of Changyuan City,Changyuan 453400,China);Clinical significance and changes of serum myocardial enzyme spectrum in children with rotavirus enteritis[J];Journal of Xinxiang Medical College;2007-04
9 CHEN Yao1,YAO Ying-min2. (1.Postgraduate Group 1,Southern Medical University,Guangzhou 510515,China; 2. Paediatrics Department,Nanfang Hospital affiliated to Southern Medical University,Guangzhou 510515,China);Progress of the Studice on the Extraintestinal Infection of Rotavirus[J];Medical Recapitulate;2006-03
10 YAO Ying-Min,LI Ning,OU Qiao-Qun,et al.Department of Pediatrics,Southern Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China.;The study of extra intestinal rotavirus infection[J];Maternal and Child Health Care of China;2008-06
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Yao Yingmin, Zhao Feng, Wu Jianchun, et al. Nanfang Hospital, Guangzhou 510515;A case report of systemic infection caused by rotavirus[J];Chinexe Journal of Pediatrics;1997-07
2 Fu Wanhai, Hao Wei, Peng Yijun. Department of Pediatrics of Zhujiang Hospital. First Military University, Guangzhou 510282;Detection of rotavirus RNA using DIG labelled probe prepared by polymerase chain reaction[J];CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY;1998-01
3 Song Haijing, Gu Zhiyuan, Lu Yanping. Molecular Biology Laboratory, PLA General Hospital Beijing 100853;Detection of human cytomegalovirus DNA in brain tissues embedded in paraffin by in situ polymerase chain reaction[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1997-06
4 Zhou Yuanping 1 Peng Wenwei 1 Yao Jilu 1 Zhang Zhigang 2 Luo Guanghan 3 Deng Zhide 1 Gu Xiuhua 1 (1 Department of Infectious Diseases, 2 Pathology, Third Affiliated Hospital, Sun Yat sen University of Medical Sciences, Guangzhou, 510630,;DETECTION ON HCV RNA IN PARAFFIN EMBEDDED LIVER TISSUES BY IN SITU POLYMERASE CHAIN REACTION[J];ACADEMIC JOURNAL OF SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;1997-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Depart ment of Western Medicine,Anhui Traditional Chinese Medical school ZHOU Feng -cang ,JIN Ming;Progresses in tumor molecular diagnosis and is study[J];Journal of Anhui Health Vocational & Technical College;2003-01
2 Anhui Provincial Children's Hospital,Hefei 230051 WEI Wen;Clinical analysis of 156 patients with rotavirus infection[J];Journal of Anhui Health Vocational & Technical College;2004-04
3 XU Liang-guo, YAO Kai-tai, XIE Lu, et al. ( Key Laboratory of Carcinogenesis Mechanism of Ministry of Health, Hunan Medical University, Changsha 410078, P. R. China School of Life Science, Zhongshan University, Guangzhou 510275, P. R. China);Comparison of different methods for in situ detection of Epstein-Barr virus in nasopharyngeal carcinoma[J];CHINESE JOURNAL OF CANCER;2000-01
4 CHEN Ai-zhen,HE Li-hong (Department of Pediatrics,Foshan First People's Hospital,Guangdong 528200,China);Changes of serum myocardial enzyme in infants with rotavirus enteritis[J];Journal of Bengbu Medical College;2005-01
5 WANG Ming Li TANG Jiu Lai HU Wen SHI Bai Fen HU Yong BI Ke Ju LI Jing Pei (Department of Microbiology, Anhui Medical University, Hefei 230032, China);DEVELOPMENT OF A MOUSE MODEL OF HUMAN CYTOMEGALOVIRUS INFECTION FOR HUMAN CONGENITAL CENTRAL NERVOUS SYSTEM[J];ACTA ZOOLOGICA SINICA;2000-02
6 Luo Yanfen,Guan Dehua,Wang Lixia (Beijing Children's Hospital,Capital University of Medical Sciences,Beijing 100045);The study of serum myocardial enzymogram in 187 children patients with infective diarrhea[J];Beijing Medical Journal;2001-05
7 LIU Chun-yi , SUN Yi , LANG Xin-ling, et al . Bao'an Maternal and Children Health Care of Hospital , Shenzhen 518133 , China;The Clinical Analysis of 108 Cases of Rotavirus Enteritis United with Hepatic Lesion[J];Journal of Chinese Physician;2005-S1
8 LI Ning, YAO Ying-min, Wen Shu-juan (Department of Paediatrics, Department of Endocrinology, Nanfang Hospital, First Militia Medical University, Guangzhou 510515, China);Viremia in infants with rotavirus diarrhea: a preliminary study[J];Journal of First Military Medical University;2001-04
9 WU Bin, LIU Xiao-li, XUAN Wen-lan, FENG Ru, YIN Fang, ZHOU Shu-yun Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Detection of stem cell factor mRNA expression in leukemic cells by in situ reverse transcriptase-PCR[J];Journal of First Military Medical University;2002-06
10 LI Ning 1 ,YAO Ying-min 1 ,WANG Zhi-yuan 2 Department of Pediatrics, Nanfang Hospital 1 , Department of Biomedical Engineering 2 , First Military Medical Univer-sity, Guangzhou 510515, China;Viremia and extraintestinal infections in infants with rotavirus diarrhea[J];Journal of First Military Medical University;2003-07
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Li-juan LIU Yu-qin(Department of Pharmacy,Shenzhen Hospital,Peking University,ShenZhen 518036,China);Curative effect of ganciclovir combined with cimetidine on infants with rotavirus enteritis[J];Anhui Medical and Pharmaceutical Journal;2009-03
2 FANG Zhao-yin~1,ZHANG Li-jie~1,TANG Jing-yu~2,ZHANG Qing~1,HU Hai-kuan~2, XIE Hua-ping~1,ZHENG Li-shu~1,Duncan Steele~3,Paul Kilgore~4,Joseph Bresee~5,Erik Hummelman~5,Xi Jiang~6,Roger Glass~5(1.Viral Gastroenteritis Division,National Institute for Viral Disease Control and Prevention,China CDC,Beijing,100052,China;2.Lulong County Epidemic Prevention Station, Hebei,Lulong 066400,China;3.World Health Organization,Switzerland;4.Division of Translational Research,International Vaccine Institute,Seoul,Korea;5.Respiratory and Enteric Viruses Branch,Division of Viral and Rickettsial Diseases,CDC,Atlanta,USA;6.Cincinnati Children's Hospital Medical Center,OH,USA.);Rotavirus Diarrhea among Children in Lulong County,Hebei Province,China[J];Chinese Journal of Virology;2005-01
3 Qian Yua Yuan Lijuan Xiong Chaohui Zhang You Liu Jun Guan Dehua Wang Zhiliang(Capital Institute of Pediatries,Beijing ,100020);IDENTTFICATION OF ROTAVIRUS G9 TYPE FROM A STOOL SPECIMEN COLLECTED FROM A CHILD WTTHDIARRHEA IN BEIJING[J];CHINESE JOURNAL OF VIROLOGY;1994-03
4 Luo Yanfen,Guan Dehua,Wang Lixia (Beijing Children's Hospital,Capital University of Medical Sciences,Beijing 100045);The study of serum myocardial enzymogram in 187 children patients with infective diarrhea[J];Beijing Medical Journal;2001-05
5 Lu Lan,Hu Lan,Wang Junling,Zhao Xiaojun (Department of Pediatrics,The First Clinical College of Hubei Medical University);Study on detection of antirotaviral antibody in human milk with ELISA method[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1999-04
6 LI Ning 1, YAO Ying Min 1,WANG Zhi Rui 2 1Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China; 2Department of Pharmacon, Xijing Hospital, Fourth Military Medical University;Co-relative factors between heart lesion and viremia in young children with rotavirus diarrhea[J];Journal of The Fourth Military Medical University;2001-08
7 YUAN Rong 1, WANG Chen-Hong 1, LIU Xiao-Mei 2, WANG Xiao-Qing 2 1Department of Gynecology, Shenzhen Municipal Maternal and Children Hospital, Shenzhen 518028, China, 2Department of Gynecology, Nanjing Municipal Maternal and Children Hospital, Nanjing 210004, China;Causes and mechanisms of immunization failure in newborns deduced by maternal-fetal hepatitis B virus (HBV) from transmission[J];Journal of The Fourth Military Medical University;2005-07
8 LI Ning, YAO Ying-min, Wen Shu-juan (Department of Paediatrics, Department of Endocrinology, Nanfang Hospital, First Militia Medical University, Guangzhou 510515, China);Viremia in infants with rotavirus diarrhea: a preliminary study[J];Journal of First Military Medical University;2001-04
9 YAO Ying-min, OU Qiao-qun, LI NingDepartment of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Extraintestinal dissemination of rotavirus in immunodeficient mice[J];Journal of First Military Medical University;2004-11
10 CHEN Yidan,XU Xuan.Department of Pediatrics,The Third Affiliated Hospital,Medical College of Jinan University,Zhuhai 510000,Guangdong;Retrospective study for rotavirus renal damage[J];Clinical Medicine;2005-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Zhao Sheng,Wang Jian Anhui Province Children's Hospital,Hefei 230051;Observation on the main biochemical markers of heart,liver and kidney in children with rotavirus enteritis[J];Anhui Medical Journal;2007-05
2 LI Hong~1,ZHANG Fei~(2)(1.Department of Pediatrics,2.Department of Pharmacy,The First People's Hospital of Suzhou,Suzhou,Anhui Province 234000);Curative effect of promethazine on rotavirus enteritis in infants[J];Anhui Medical and Pharmaceutical Journal;2006-09
3 Li Ning Ou Qiaoqun~① Dai Jing~② et al.Nanfang Hospital,Guangzhou 510515,China ①Gerneral Hospital of Guangzhou Military Area Command,Guangzhou 510010,China. ②Changhai Hospital,Second Military Medical University,Shanghai 200433,China;Exploring the distribution of rotavirus receptors in human organs[J];Infectious Disease Information;2008-04
4 CHEN Jun-hua,XIE Yuan-yuan,ZHU Chao-min,LIU Zuo-yi(Department of Infectious Disease,Children's Hospital,Chongqing Medical University,Chongqing 400014,China);Extraintestinal lesions caused by rotavirus:An experimental study on suckling mice[J];Acta Academiae Medicinae Militaris Tertiae;2009-15
5 CHEN Chao1,2,GAO Hong2,ZHAO Bao-hong2,QU Chang-lin2 (1.Ruili Drug Detector Dog Base of China Custom,Ruili,Yunnan,678600,China;2.College of Animal Science and Technologe,Yunnan Agriculture University,Kunming,Yunnan,650201,China);In-situ PCR and Its Application in Pathology[J];Progress in Veterinary Medicine;2007-05
6 SUN Yi-yang(No.3 People's Hospital of Honghe Autonomous Prefecture,Yunnan Province,Yunnan Gejiu 661000,China);The Observation of Curative Effect of Interferonα-2b Rectum Enema on 86 Cases of Autumn Infantile Diarrhea[J];Journal of Pediatric Pharmacy;2010-03
7 LIN Cai,LI Qian,MO Wei-xiong,LI Nan-yang,ZHANG Ming-zhen,YANG Mei-xia.The first people's hospital of Zhaoqing,Zhaoqing 526021,China;Clinical study of the myocardial damage and liver damage with rotavirus enteritis in infants[J];International Medicine & Health Guidance News;2010-02
8 HAN Dong-mei,YU Li-ying(The People's Hospital of Langfang ,Hebei Province,Langfang 065000,China);Investigation effects of injective Yanhuning with powdered milk in the treatment of rotavirus enteritis in infantile[J];The Chinese Journal of Clinical Pharmacology;2008-01
9 LI Bo,FENG Li-qiong,CHEN Ze-qi,WANG Jun-fu(Department of Pediatrics,Songgang Hospital of Nanhai District,Fushan City,Fushan 528234,China);Effects of treating infantile rotavirus enteritis with potassium sodium dehydroandroan drographolide succinate and cemitidine[J];Journal of Hainan Medical College;2008-03
10 LIN Tao,DENG Jin-you,XIAO Li,JIE Dong-ying(Pediatric Department of Lianjiang People's Hospital Zhanjiang 524400,China);Effect of zinc gluconate tablets on serum zinc and diarrhea of children with rotariras enteritis[J];Journal of Hainan Medical University;2010-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Fu Wan-hai,Peng Xuan-jun,zhuo Li-mei,et al(First Military Medical U niversity Zhujiang H ospital,Guangzhou 510282);DETECTION OF ROTAVIRUS FROM ASYMPTOMATIC NEONATAL INFECTION WITH POLYMERASE CHAIN REACTION[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1994-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved