Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Obstetrics And Gynecology》 2000-08
Add to Favorite Get Latest Update

Detecting Embryo Sex by Fluorescence In-situ Hybridization in Preimplantation Genetic Diagnosis

XU Yanwen, ZHUANG Guanglun, LI Man, et al. Reproductive Medical Research Center, Department of Obstetric and Gynecology, First Affiliated Hospital of Sun Yat sen University of Medical Sciences, Guangzhou 510080, China  
Objective Applying fluorescence in situ hybridization(FISH) in preimplantation gender diagnosis. Methods Ovarian hyperstimulation was performed in 2 hemophilia A carriers and 2 patients with Y chromosome abnormality. Embryo sex was identified by single blastomere FISH after embryo biopsy. Female embryos were transferred into uterus. Results A total of 110 cumulus oocyte complex were retrieved in 5 treatment cycles. Among them, 68.2% showed normal fertilized pronuclei and 55 embryos were available for embryo biopsy. The success rate of biopsy was 85.5%, with further cleavage rate of 61.7%. In FISH procedure, one cell was lost during fixation, led to 97.9% fixation rate. Totally, 18 female embryos were diagnosed and 16 were transferred into uterus. Two clinical and one biochemical pregnancies were achieved. The diagnosis was confirmed by the following analysis of amniocyte and embryonic buds after embryo reduction respectively. Conclusions FISH is an efficient and accurate technique for determination of the sex of human preimplantation embryos. Selective abortion and birth of affected child can be avoided by preimplantation gender diagnosis.
【CateGory Index】: R714.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ruo-peng, ZHANG Li-rong, DAO Cheng-Ian et al (Reproductive Center, The Affiliated Hospital of Dali University, Dali,Yunnan 671000, China);Research Advance of Preimplantation Genetic Diagnosis[J];Journal of Dali College;2005-01
2 Deng Mingfen,Lin Wenqing,Luo Chunqi,Li Suiping.(The First Hospital Affiliated to Zhongshan University,Guangzhou,510080,China);Nursing on Patients with Preimplantation Genetic Diagnosis[J];Heilongjiang Nursing Journal;2005-19
3 YAN Jie,TIAN Qing-hua.Clinical Medical School of Luzhou Medical College,Luzhou 646000,China;Prevent Abusing of Assisted Reproductive Technology: Respect for Women's Demands[J];Medicine & Philosophy(Humanistic & Social Medicine Edition);2011-07
4 ZHUANG Guang-lun;The history and development of assisted reproductive technologies[J];Chinese Journal of Practical Gynecology and Obstetrics;2010-10
5 XU Yan-wen;The challenge of preimplantation genetic diagnosis technology[J];Chinese Journal of Practical Gynecology and Obstetrics;2010-10
6 JIAO Zexu,ZHUANG Guanglun. (Reproductive Medical Center, First Affiliated Hospital, Sun Yat-Sen University Sciences,Guangzhou, Guangdong, 510080 P.R.China.;Preimplantation genetic diagnosis[J];Chinese Journal of Medical Genetics;2002-03
7 FANG Cong, ZHUANG Guang lun, XU Yan wen, SHU Yi min, ZHOU Can quan, LI Jie, Zhong Yi ping (Reproductive Medical Center, First Affiliated Hospital of Sun Yat sen University of Medical Sciences, Gangzhou 510080, China);Preimplantation Genetic Diagnosis for Robertsonian Translocation Carrier[J];Academic Journal of Sun Yat-sen University of Medical Sciences;2001-03
8 Ning Yuling,Li Lisha,Ren Hongjin. (The Reproductive and Infertility Institute of Chengdu's Gynecolog and Obstetrics Hospital,610015);The analysis about genetics pathogenesis for the patient of primary azoospermatism[J];Chinese Journal of Birth Health & Heredity;2002-01
9 XU Zheng-feng,HU Ya-li,CAI Mei-yan, et al.(Prenatal Diagnosis Center,Drumtower Hospital,Medical School,Nanjing University,Nanjing,Jiangsu 210008);An improved fixation technique of blastomeres for fluorescence in situ hybridization for preimplantation genetic diagnosis[J];Chinese Journal of Birth Health & Heredity;2004-01
10 DOU Rong,et al. (Shang hai Changning District Center Hospital,Shanghai,200336,China);Relationship analysis between abnormal karyotype of venule blood chromosome and clinical diagnosis in 120 cases[J];Chinese Journal of Birth Health & Heredity;2004-06
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Zhuang Guanglun,et al.First Affilialed Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou,510080;Human in vitro fertilization and embryo transfer:a report of 34 cases[J];Journal fo Reproductive Medicine;1992-01
2 XU Yanwen, ZHUANG Guanglun, ZHOU Canquan, et al. Reproductive Medical Center, The First Affiliated Hospital of Sun Yat sen University of Medical Sciences, Guangzhou 510080;Dual Color Fluorescent In situ Hybridization Detects Sex Chromosome Mosaicism in Human Embryos[J];Chinese Journal of Obstetrics And Gynecology;1999-10
3 LiRong,ZhuangGuanglun,ZhouCanquan.FirstAfiliatedHospitalofSunYet-SenU-niversityofMedicalSciences,Guangzhou510080;FertilizationinVitroandEmbryoTransferbyCombinedLong┐ActingGonadotropinReleasiningHormoneAgonistandGonadotropinforSuperovulationinPatientswithRefractoryPolycysticOvarySyndrome[J];Chinese Journal of Obstetrics And Gynecology;1996-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Yan-wen~Δ ZHUANG Guang-lun (Reproductive Medical Center,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China);The latest development in preimplantation genetic diagnosis[J];Journal of Peking University(Health Sciences);2004-06
2 (ZHU Shao-hua, FISCHER FRAUKE/Department of Forensic Medicine of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China);Detection of chromosome X and Y by two - color fluoresence in situ hybridization in human blood and bloodstain for sex identification[J];Chinese Journal of Forensic Medicine;2004-03
3 Deng Mingfen,Lin Wenqing,Luo Chunqi,Li Suiping.(The First Hospital Affiliated to Zhongshan University,Guangzhou,510080,China);Nursing on Patients with Preimplantation Genetic Diagnosis[J];Heilongjiang Nursing Journal;2005-19
4 ZHANG Xiao yan, CHEN Wen zhen, KAN Yao fan, et al (Fujian Provincial Maternity and Children Health Hospital, Fuzhou 350001,China);Study on the Optimal Scheme of Ovarian Hyper-stimulation in IVF-ET Cycles[J];Strail Journal of Preventive Medicine;2001-03
5 Feng Liang,Wang Gongxian,Cheng Weiming,et al.(The first Afflicated hospital of JiangXi medical, col-lege,NanChang,330006);Intracytoplasmic Injection Using Sperm Aspirated from the Epididymis or Testis for Treatment of Obstructive azoospermia[J];Jiangxi Medical Journal;2002-04
6 JIAO Zhe xu,ZHUANG Guan lun,ZHOU Can quan,et al (Reproductive Medical Center,The First Affiliated Hospital of Sun Yet Sen University of Medical Sciences,Guangzhou 510080);Monozygotic Twinning and In Vitro Fertilization and Embryo Transfer:a Reprot of 4 Cases and Review of the Literature[J];Journal of Practical Obstetrics and Gynecology;2001-06
7 DONG Yan, WU Dan, XU Qing-wen, ZHANG Chen, PING Yuan, ZHOU Huai-gu(Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security, Shanghai 200083, China);Applications of Fluorescence in Situ Hybridization(FISH) in Forensic DNA Analysis[J];Chinese Journal of Forensic Sciences;2009-04
8 JIA Xueling,GU Chongjuan,SHI Xin,et al (The Reproductive Medicine Research Center,The First Hospital of Lanzhou University,Lanzhou Gansu 730000,China);The Influences of Aspirating Big Follicles to the IVF-ET Pregnancy Outcome of the Follicles Develop Non-synchronously Patients in Vitro Fertilization-Embryo Transfer GnRH-a Long Protocol[J];Journal of Practical Obstetrics and Gynecology;2013-02
9 Meng Yushi, Zhan Xiaomei, Li Bo, et al. Yunnan Provincial First People's Hospital, Kunming.(650032);Human in vitro fertiliyation and embrgo transper of 16 cases.[J];MEDICINE AND PHARMACY OF YUNNAN;1999-05
10 Xu Yanwen, Zhuang Guanglun, Zhou Canquan, et al. Reproductive Medical Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510080,China;Morphological analysis of transferred embryos in successful in vitro fertilization and embryo transfer treatment cycles[J];Chinese Journal of Practical Gynecology and Obstetrics;2001-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LU Yeling,DING Qiulan,DAI Jin,et al.Department of Transfusion,Ruijin Hospital,Shanghai 200025,China;Carrier detection and prenatal diagnosis of haemophilia[J];Chinese Journal of Blood Transfusion;2008-04
2 Shi-xiu LIAO 1* , Fei-fei HUANG 2, Yan-li YANG 2, Zi-bo SONG 2, Ying-tai WANG 2, Zhao-cai WANG 2, Xian-zhong QU 3, Han-ping CHEN 1△ (1.Department of Gynecology and Obstetrics,Affiliated Tongji Hospital,Tongji College,Huazhong University of Technology,Wuhan 430030,China;2.Medical Genetics Institute ,the People’s Hospital of Henan Province;3. Department of Ultrasound, the People’s Hospital of Henan Province);Screening for chromosomal abnormalities using nuchal translucency measurement with materal serum biochemistry markers in first trimester[J];Journal of Peking University(Health Sciences);2005-01
3 LIANG Meiying,CHEN Junli,SONG Guining. (Peking University People′s Hospital,Beijing 100044,China);To analyse the relationship between large Y chromosome and abnormal pregnancy[J];Chinese Journal of Clinical Obstetric and Gynecology;2005-02
4 XIAO Xiao-su,LIU Xiao-yi,WANG Yong-qiang,HU Fei-xue,YANG Yuan-hui.(Laboratory of Genetics,Department of Laboratory,Beijing University Shenzhen Hospital,Shenzhen 518036,P.R.China)Corresponding author: XIAO Xiao-su.E-mail: xiaosu-xiao@yahoo.com.cn;Genetic Effect of Pericentric Inversion of Chromosome 9[J];International Journal of Genetics;2006-02
5 WU Yue-li, WU Ling-qian, LI Yan-ping, LIU Dong-e, ZENG Qiao, ZHU Hai-yan, PAN Qian,LIANG De-sheng, HU Hao, LONG Zhi-gao, LI Juan, DAI He-ping, XIA Kun, XIA Jia-hui(National Laboratory of Medical Genetics, Xiangya Hospital, Central South University, Changsha 410078, China);Preimplantation genetic diagnosis of Duchenne muscular dystrophy by single cell triplex PCR[J];Journal of Central South University(Medical Sciences);2007-02
6 LUO Xin-jing 1, MO Xuan-rong 1, XU Ke-qian 2 (1. Faculty of medical laboratory, Medical College of Taizhou University, Zhejiang Taizhou 318000, China; 2. Faculty of Laboratory, Xiangya Medical College of Zhongnan University, Hunan Changsha 410078, China);Detection of circulating fetal DNA in maternal plasma and its application in prenatal diagnosis[J];Foreign Medical Sciences Section of Maternal and Child Health;2004-01
7 JIA Yi-fang,WU Ai-hua,ZHANG Ai-dong (Key Laboratory of Eugenic Technique, Shandong Provincial Research Institute for Family Planning,Shandong Jinan 250002,China);Progress in study on fetal cell-free nucleic acid in maternal peripheral blood[J];Foreign Medical Sciences Section of Maternal and Child Health;2004-05
8 YANG Mingjie, CAO Jia(Molecular Toxicology Laboratory, Third Military Medical College,Chongqing 400038, China).;Principle and Applications of Multicolor Fluorecsence in situ Hybridization Technology.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1998-04
9 ZHANG Yueping, HAN Jinlan, REN Delin. Shanghai JiAi Genetics and IVF Institute, Shanghai, 200011;Analyzing karyotype of abortus conceived after in vitro fertilization-embryo transfer ( IVF- ET )[J];Shanghai Medical Journal;2002-08
10 Dong-sheng TANG, Xiao-jie LI Zhi-hua CHEN , Zhen-cheng LIN Guang YANG, Xiao-ling GUO (Foshan University , Foshan, 528000);Chromosome Heteromorphism and Early Reproduction Inability[J];Reproduction and Contraception;2003-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 XU Yan-wen~Δ ZHUANG Guang-lun (Reproductive Medical Center,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China);The latest development in preimplantation genetic diagnosis[J];Journal of Peking University(Health Sciences);2004-06
2 REN Meihong;ZHANG Xiaohong;SONG Guining;ZHANG Lin;Prenatal Diagnosis Center,Peking University People's Hospital;;Study on the clinical application of FISH in prenatal diagnosis and detection of abortion tissue[J];Chinese Journal of Clinical Obstetrics and Gynecology;2013-05
3 Deng Mingfen,Lin Wenqing,Luo Chunqi,Li Suiping.(The First Hospital Affiliated to Zhongshan University,Guangzhou,510080,China);Nursing on Patients with Preimplantation Genetic Diagnosis[J];Heilongjiang Nursing Journal;2005-19
4 ZOU Qi~1,SUN De-gui~2,ZHAO Hui~2 (1.Nursing College of Zhengzhou University;2.The Third Affiliated Hospital of Zhengzhou University 450002,China);Cytogenetics Research of Couples with Abnormal Procreation[J];Journal of Henan Medical College For Staff and Workers;2005-06
5 Wan Junhui1,Zhong Zeyan1,Tian Peiling2,Wei Xiangcai2,*,Zhang Qingjian2,Li Mingzhen2 1.Department of Genetics,Southern Medical University,Guangzhou 510515;2.Family planning Research Institute of Guangdong;Application of fluorescence in situ hybridization for prenatal diagnosis and pre-implantation genetic diagnosis of Down syndrome[J];Chinese Journal of Family Planning;2011-09
6 LI Qiang, SUN Yu-li, SUN Yu-ping, et al (Reproduction Medical Center, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China);;CYTOGENETICAL ANALYSIS OF PATIENTS WITH PRIMARY AZOOSPERMIA[J];Medical Journal of Qilu;2005-01
7 WU Qing-ming1,ZHOU Jin2,LIU Wei1,WANG Rui3,YANG Liu3,YU Liang-kuai 2 (1 Medical College of Shihezi University Shihezi, Xinjiang 832000, China 2 Department of Obstetrics&Gynecology, Urumuqi General Hospital of Lanzhou Military region, Urumuqi 830000, China 3 Station of cliniccal research, Urumuqi General Hospital of Lanzhou Military region, Urumuqi 830000, China);Result Analysis of 1641 Prenatal Screening and 95 Prenatal Diagnosis in Second Trimester[J];Progress in Modern Biomedicine;2010-14
8 DUAN Cheng-ying,WANG Li-juan,WANG Ting,Ou Jian,ZHONG Hong-ling,FU Wen-yu,ZHANG Jing,WANG Wei-wei,LI Hong(Center of Reproduction and Genetics,Suzhou Municipal Hospital,Suzhou Hospital Affiliated to Nanjing Medical University,Jiangsu Suzhou 215002,China);Analysis of Abnormal Chromosome of Genetic Insult Patients in Suzhou[J];Suzhou University Journal of Medical Science;2010-01
9 Cheng-ying DUAN,Li-juan WANG,Ting WANG,Jian OU,Wen-yu FU,Hong-ling ZHONG,Jing ZHANG,Hong LI(Center of reproduction and Genetics,Suzhou Municipal Hospital,Affiliated Suzhou Hospital,Suzhou,215002);Relationship between Clinical Reproductive Abnormalities and Chromosome Polymorphisms[J];Reproduction & Contraception;2009-10
10 Xiao-hong GUAN,Ai-jun ZHANG,Yi-juan SUN, Xiao-wei LU,Rui-huan GU,Yun FENG(Reproductive Medical Center,Ruijin Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai,200025);Using Trophectoderm Biopsy to Analyze the Development and Chromosomal Abnormalities of Blastocysts Derived from Day 3 Discarded Embryos[J];Reproduction & Contraception;2011-02
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhuang Guanglun,et al.First Affilialed Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou,510080;Human in vitro fertilization and embryo transfer:a report of 34 cases[J];Journal fo Reproductive Medicine;1992-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved