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《Chinese Journal of Hepatobiliary Surgery》 2001-11
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Isolation and establishment of human hepatocellular carcinoma cell clones with various metastatic potentiais

LI Yan TANG Zhaoyou YE Shenglong,Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai 20032,P.R.China  
Objective To establish hepatocellular carcinoma(HCC)cell clones with various me- tastatic potentials from highly metastatic HCC cell line MHCC97.Methods Cell clone culture was conducted on the 25th passage of the source cell line MHCC97 using limited dilution method.Target cell clones were selected by in vivo screening in nude mice.Biological characteristics of the target clones were studied in terms of tumor cell doubling time,karyotyping,in vitro invasiveness,alpha-fe- toprotein secretion and pulmonary metastasis after orthotopic implantation.Results HCC clones with high(MHCC97-H)and low(MHCC97-L)metastatic potentials were established from the source cell line MHCC97.When MHCC97-H was compared with MHCC97-L,the pulmonary metastatic rate was 100%(10/10)versus 40%(4/10),tumor cell doubling time 34.2 hours vs.60.0 hours,and Boyden chamber in vitro invasion assay was 37.5±11.0 cells/field vs.17.74±6.3 cells/field.Karyotyping re- vealed that the main image of chromosomes was 55—57(68%)in MHCC97-H and 57—62(58%)in MHCC97-L.The proportions of cells in G_0—G_1 phase,S phase,and G_2-M phase for MHCC97-H/ MHCC97-L were 56.10%/65.32%, 28.35%/24.96% and 15.64%/9.73%,respectively,as meas- ured by flow cytometry.The serum AFP levels in nude mice in 5 weeks after orthotopic implantation of tumor tissue were 245.8±66.1μg/L for MHCC97-H and 91.2±65.7μg/L for MHCC97-L.Con- clusions The results confirm the heterogeneity of MHCC97.These tow clones of the same genetic background are of great value for further investigation on metastasis of HCC.
【Fund】: 国家重点基础研究发展规划“恶性肿瘤发生与发展的基础性研究”G1998051211 ;; 上海市医学领先专业重大项目:“肝癌转移的分子机制及干预治疗的实验研究”983001
【CateGory Index】: R735.7
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Chinese Journal Full-text Database 7 Hits
1 Liu Hu,Ye Sheng -Long ,Li Wen-Chang ,Li Yan,Liu Yin-Kun,Qin Lun-Xiu,Qiu Shuang -Jian,Sun Rui-Xia,Chen Jie,Tang Zhao-YouLiver Cancer Institute /Zhongshan Hospital,Fudan University,Shanghai,200032,P.R.China;Quantitative Analysis of Expression of TEY1 Gene in Liver Cancer Cells with Different Metastatic Potential[J];Chinese Journal of Cancer;2003-06
2 Cao Yaling1,Chen Ling1,Lu Muhan1,Wei Xiaolin2,Wu Yuyun1,Luo Gang1,Liu Xiangde2,Yang Shiming1(1Institute of Gastroenterology,2Institute of Hepatobiliary Surgery,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Expression of miR-149-5p in hepatocellular carcinoma and its clinical significance[J];Journal of Third Military Medical University;2012-07
3 LI Hong-tao1,LIANG Hou-jie1,LI Wen-mei2,XIAO Wen-hua3 1.Department of Oncology,Southwest Hospital,Third Military Medical University,Chongqing 400038,P.R.China2.Laboratory of Molecular Oncology,Beijing Institute for Cancer Research,School of Oncology,Peking University,Beijing 100141,P.R.China3.Department of Oncology,First Affiliated Hospital of General Hospital of PLA,Beijing 100037,P.R.China;Expressions of three DNA methyltransferases and classⅠHLA in hepatocellular carcinoma cells[J];Chinese Journal of Cancer Prevention and Treatment;2010-15
4 Wei GU,Ya-Ni ZHANG,Bai LI,Jie HAN,Bin-Bin CHENG,ChangQuan LING(Department of Traditional Chinese Medicine,Changhai Hospital,Second Military Medical University,Shanghai 200433,China);Establishment of a multidrug-resistant cell line BEL-7402/5-FU of human hepatocellular carcinoma and its biological characteristics[J];Journal of Chinese Integrative Medicine;2006-03
5 SUN Jian, WU Zhiquan, QUE Qiong, et al. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China;An experimental study on inhibition of lung metastasis of hepatocellular carcinoma by trichosanthin[J];Chinese Journal of Hepatobiliary Surgery;2005-04
6 LI Li-xin, YE Sheng-long, WANG Yan-hong, et al. (Liver Cancer Institute, Zhongshan Hospital, Fudan University, Clinical Medicine Centre for Liver Cancer, Shanghai 200032 ,China);Metastatic Potential Inhibited with Trametes Robiniophila in Highly Metastatic Potential Human Hepatocellular Carcinoma Cells Lines[J];Bulletin of Chinese Cancer;2006-04
7 LI Yan, TANG Zhaoyou, YE Shenglong, LIU Yinkun, CHEN Jie, XUE Qiong, HUANG Xiaowu, CHEN Jun, BAO Weihua, YANG Jiong, GAO Dongmei. Liver Cancer Institute and Zhong Shan Hospital of Fudan University, Shanghai, 200032, China;Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection[J];National Medical Journal of China;2002-09
Chinese Journal Full-text Database 10 Hits
1 WONG Yick-fu WONG Wu-shun CHDANG M Z (Department of Obstetrics & Gynaecology y, Chinese University of Hong Kong)YEUNG Hin-wing (Departmeunt of Biochemistry , Chinese University of Hong Kong);INHIBITORY EFFECT OF CRYSTALLINE TRICHOSANTHIN ON THE GROWTH OF TUMOUR CELLS IN VITRO[J];Chinese Journal of Cancer;1989-03
2 TU Shui ping* , JIANG Shi hu, QIAO Min min, WANG Li fu, ZHANG Shu, YUAN Yao zong, WU Yun li,WU Yu xin. Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025,P.R.China;Induction of apoptosis by trichosanthin in gastric cancer cell MKN-45[J];Chinese Journal of Cancer;2000-12
3 Yang Li Hua1, Wang Kai Feng2, Zhou Yi Ping2, Li Ma Lin2 1.Department of Obstetric and Gynecology,Second Affiliated Hospital,Kunming Medical College, Kunming,Yunnan,650101, P.R.China 2.Yunnan Pharmacological Laboratory of Natural Product, Kunming,Yunnan,650101, P.R.China;Role and Mechanism of Nuclear Factor κB in Angiogenesis of Human Ovarian Carcinoma[J];Chinese Journal of Cancer;2004-05
4 ZHANG A-Li1, WANG Quan-Sheng2, ZHONG Ya-Hua1, CHEN Gang3, XI Ling3, XIE Cong-Hua1, ZHOU Yun-Feng1, MA Ding3 1. Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, 430071, P. R. China 2. Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China 3. Department of Gynecology and Obstetics Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China;Effect of Transcriptional Factor Snail on Epithelial-Mesenchymal Transition and Tumor Metastasis[J];Chinese Journal of Cancer;2005-11
5 ZHANG Jun-Ping1, PAN Hong-Ming1, FANG Yong1, HUANG Li-Peng2, WU Jin-Min11. Department of MedicalOncology,Sir Run Run Shaw Hospital,Zhejiang University,Hangzhou, Zhejiang, 310016,P. R. China2. Department of The FirstSurgery,Luoyang Shaft Bearing ManufactoryHospital,Luoyang, Henan, 471003,P. R. China;Impact of Radiofrequency on Splenocyte Immunity of Mice Bearing H22 Liver Cancer[J];Chinese Journal of Cancer;2006-01
6 ZHOU Qi-Ming1,2, WU Pei-Hong1,2, ZHAO Ming1,2, WANG Qi-Jing1,3, HUANG Li-Xi1,3, LI Yong-Qiang1,3, CHEN Shi-Ping1,3, XIA Jian-Chuan1,31. State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China2. Department of Imaging and Interventional Radiology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China 3. Department of Biotherapy, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China;Short-term Curative Efficacy of Cytokine-induced Killer Cells Combined Micro-invasive Treatments on Hepatocellular Carcinoma[J];Chinese Journal of Cancer;2006-11
7 Wang Lu # Tang Zhaoyou Liver Cancer Institute & Zhongshan Hospital,Fudan University,Shanghai 200032;Establishment of the metastasis and recurrence nude mice models and cell line of human hepatocellular carcinoma and application of models to experimental intervention[J];Oncology Progress;2005-01
8 Zhang Boheng 1,2# Tang Zhaoyou 1 Yu Yue 1 Wu Zhiquan 1 Fan Jia 1 Qin Lunxiu 1 Zhou Jian 1 Sun Huichuan 1 Qiu Shuangjian 1 Ye Shenglong 1 Ren Zhenggang 11 Liver Cancer Institute of Fudan University 2 Evidence-Based Medicine Center,Shanghai 200032,China;Relevant factors to long-term survival after surgical treatment of hepatocellular carcinoma[J];Oncology Progress;2005-01
9 Sao Chunkui; Xiong Min(Department of Pathology, Bengbu Medical College,Anhui 233003);Study on morphological and metastatic properties of three B_(16) melanoma cell strains[J];ACTA ACADEMIAE MEDICINAE BENGBU;1996-01
10 Yang Xinke Chen Zhangliang Duan Shumin Li Yuying Wu Shuhua Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering Beijing National Laboratory of Plant Molecular Biology, Faculty of Biology, Beijing University;ANTIVIRAL ACTIVITIES OF TKP ISOLATED FROM TRICHOSANTHES KIRILOWII[J];Chinese Journal of Virology;1990-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Liu Hu,Ye Sheng -Long ,Li Wen-Chang ,Li Yan,Liu Yin-Kun,Qin Lun-Xiu,Qiu Shuang -Jian,Sun Rui-Xia,Chen Jie,Tang Zhao-YouLiver Cancer Institute /Zhongshan Hospital,Fudan University,Shanghai,200032,P.R.China;Quantitative Analysis of Expression of TEY1 Gene in Liver Cancer Cells with Different Metastatic Potential[J];Chinese Journal of Cancer;2003-06
2 PENG Zhihong~1, TANG Bo~2, YANG Jianmin~1, SI Suihai~1, CHEN Wensheng~1, FANG Dianchun~1, LUO Yuanhui~1 (~1Gastroenterology Research Center, ~2Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of metastasis suppressor gene KAI1 on adhesion forces of MHCC97-H hepatocellular carcinoma cells with high metastatic potential[J];Acta Academiae Medicinae Militaris Tertiae;2005-23
3 TANG Hao1,LIANG Ping2,LI Rong-ping3 1Department of Hepatobiliary Surgery,187 Central Hospital of PLA,Haikou 571159,China; 2Department of General Surgery,3Department of Digestive Medicine,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China;Establishment of a 5-DFUR-resistant human hepatocellular carcinoma cell model and preliminary study of the mechanisms of the drug resistance[J];Journal of Southern Medical University;2008-11
4 YAN Feng1,WANG Xiao-min2,PAN Chao3 1Postdoctoral Station,2Department of Hepatobiliary Surgery,3Department of Pathology,Zhongshan Hospital,Xiamen University,Xiamen 361004,China;Expression of ERK5 in multidrug-resistant hepatocellular carcinoma cell line[J];Journal of Southern Medical University;2009-03
5 He Bing1*,Yu Songtao2*,Lyu Muhan1,Wu Yuyun1,Hu Changjiang1,Yang Shiming1(1Institute of Gastroenterology,Xinqiao Hospital,Third Military Medical University,Chongqing,400037;2Department of Oncology,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);miR-29 functions as an anti-metastatic microRNA in gastric adenocarcinoma by targeting ITGB1[J];Journal of Third Military Medical University;2013-11
6 Lv Changkun1#,Wang Pengbo2,Ma Feifei2,Luo Chunli1,Wan Xuelian2,Wang Changhai2(1.Department of Laboratory Diagnosis,Chongqing Medical University,Chongqing 400016,China; 2.Office of Educational Administration,Shangqiu Medical College,Shangqiu,Henan 4761000,China);Study on establishment of golden hamster buccal squamous carcinoma model for and its biological characteristics[J];Chongqing Medicine;2013-21
7 QUE Wen-zhong,XU Yun-lu,XIE Jie-ming(Department of pharmacy,Fujian Medical University,Fuzhou 350004,China);Pogress of antitumor effect of type Ⅰ ribosome inactivating proteins[J];Strait Pharmaceutical Journal;2009-05
8 CHEN Liangdong,LI Yan,ZHENG Fang,LI PengchengDept.of Oncology,Zhongnan Hospital of Wuhan University & Cancer Center of Wuhan University,Wuhan 430071,China;Study on the Intraperitoneal Chemotherapy with Hydroxycamptothecin for the Treatment of Peritoneal Carcinomatosis in Mice[J];Medical Journal of Wuhan University;2008-06
9 WANG Jianying,XU Qian,WU Shujuan,et al.The Forth Hospital of Hebei Medical University,Shijiazhuang 050011,China;The influence of drugs on the expression of HLA-G and HLA-E in transplanted ovarian cancer tissues of nude mice[J];Hebei Medical Journal;2009-21
10 LEI Zhan-ping,ZHU Jian-si,WANG Cheng-kun,et al(Institute of Cancer Research,Nanhua University,Hengyang,Hunan 421001,China);The Identification and Screening of the High Invasive Metastatic Strain of Mice Histiocytosarcoma L-Ⅱ[J];Journal of Nanhua University(Medical Edition);2006-03
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