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Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs

OU Long, LIU Hongwei, PANG Jinfan,et al Department of Stomatology, General Hospital of PLA, Beijing 100853,China  
Objective To evaluate the effect of autogenous periodontal ligament cell (PDL) transplantation in periodontal tissue regeneration Methods PDL cells derived from the same dog were cultured with α MEM 1×10 5 cells of first passage were allowed to attach to the collagen membrane for 24 hours The membrane cells were transplanted into periodontal defect in the same dog Then the defects were covered with e PTFE membranes The defects covered only with e PTFE were the controls Eighteen teeth of 6 dogs for each group were included The dogs were sacrificed after 6 weeks Results The results showed that new bone formation (4 00±0 13)mm in test group was significantly higher than that of in control group (3 09±0 28)mm, P 0 05 The new cementum formation in test group was better than control group ( P 0 05) Conclusion The results suggested that PDL cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs
【Fund】: 国家自然科学基金资助项目 !(39770798);; 国家教委留学回国人员科研启动基金资助项目![外教司留(1997)436]
【CateGory Index】: R780.2
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