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《Chinese Journal of Stomatology》 2005-03
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Effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts

LI Bin-bin, YU Shi-feng, PANG Shu-zhen. Department of Oral Pathology, Peking University School of Stomatology, Beijing 100081, China  
Objective To study the effect of genistein on the proliferation, differentiation and apoptosis of the osteoblasts in vitro. Methods The primary osteoblasts (OBs) were obtained from the rat calvaria and the cell line of osteosarcoma -UMR-106 served as control. The cells in the experiment group were grown in 10% (volume fraction) fetal calf serum ( FCS ) + αMEM + various concentrations of genistein. The control groups were grown in 10% FCS +αMEM. The growth of OBs was assessed by flow cytometry and MTT method. The differentiation of OBs was examined by the alkaline phosphatase(ALP) activity. Results Flow cytometry analysis and MTT showed that genistein could prompt primary OB from stage G_0(G_1) to stage S, G_2 or M. By contrast, genistein had no effect on the cell cycle of UMR-106, but could induce its apoptosis. Additionally, the results of ALP activity showed that genistein stimulated the differentiation of primary OB. Conclusions Genistein can stimulate the proliferation and differentiation of the primary osteoblasts in some degree, and induce the apoptosis of osteosarcoma cells.
【Fund】: 国家自然科学基金资助项目(30271412);; 北京大学211工程口腔医学学科群基金资助项目(KQ0221116)
【CateGory Index】: R780.2
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